DNA methylome of human peripheral blood mononuclear cells from the YH Han Chinese individual.

Dataset type: Epigenomic
Data released on November 12, 2011

Li Y; Zhu J; Tian G; Li N; Li Q; Ye M; Zheng H; Yu J; Wu H; Sun J; Zhang H; Chen Q; Luo R; Chen M; He Y; Jin X; Zhang Q; Yu C; Zhou G; Sun J; Huang Y; Zheng H; Cao H; Zhou X; Guo S; Hu X; Li X; Kristiansen K; Bolund L; Xu J; Wang W; Yang H; Wang J; Li R; Beck S; Wang J; Zhang X (2011): DNA methylome of human peripheral blood mononuclear cells from the YH Han Chinese individual. GigaScience. http://dx.doi.org/10.5524/100014


The methylome reported and analyzed here was generated from the same sample of peripheral blood mononuclear cells (PBMCs) from a consented donor (Homo sapiens) whose genome was deciphered in the YH project. YH is an anonymous male Han Chinese individual who has no known genetic diseases, and whose genome also serves as an Asian reference genome. Nuclear DNA was extracted and subjected to unbiased, whole-genome bisulfite sequencing (BS-seq) using the Illumina Genome Analyzer. In total, 103.5 Gbp of paired-end sequence data were generated. Of these, 70.4 Gbp (68%) were successfully aligned to either strand of the YH genome with an average mismatch rate of 1.3%, resulting in an average sequencing depth of 12.3-fold per DNA strand or a 24.7-fold overall depth. Of the 18,962,679 CpGs present in the unique haploid part (2.21 Gb) of the YH reference genome sequence, approximately 99.86% were covered by at least one unambiguously mapped read of quality score >14 on either strand, and 92.62% were unambiguously covered on both strands.

Additional details

Read the peer-reviewed publication(s):

Li, Y., Zhu, J., Tian, G., Li, N., Li, Q., Ye, M., … Zhang, X. (2010). The DNA Methylome of Human Peripheral Blood Mononuclear Cells. PLoS Biology, 8(11), e1000533. doi:10.1371/journal.pbio.1000533 (PubMed: 21085693)

Related datasets:

doi:10.5524/100014 IsSupplementTo doi:10.5524/100015

Accessions (data generated as part of this study):

SRA: SRP002339
GEO: GSE17972


Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
YH9606HumanhumanHomo sapiens
Displaying 1-1 of 1 Sample(s).

File NameSample IDData TypeFile FormatSizeRelease Date 
YHGenome sequenceFASTQ314.65 MB2011-11-12
YHGenome sequenceFASTQ358.9 MB2011-11-12
YHGenome sequenceFASTQ266.76 MB2011-11-12
YHGenome sequenceFASTQ262.09 MB2011-11-12
YHGenome sequenceFASTQ692.31 MB2011-11-12
YHGenome sequenceFASTQ702.46 MB2011-11-12
YHGenome sequenceFASTQ816.49 MB2011-11-12
YHGenome sequenceFASTQ933.71 MB2011-11-12
YHGenome sequenceFASTQ568.35 MB2011-11-12
YHGenome sequenceFASTQ755.84 MB2011-11-12
Displaying 1-10 of 182 File(s).
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