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Data released on May 18, 2013

Quantitative proteomics data using mTRAQ/MRM looking at human AKR family members in cancer cell lines

Cha, C; Lin, L; Liu, S; Lou, X; Qiu, X; Sun, H; Wang, Q; Wen, B; Xu, S; Yang, L; Zhang, S; Zhang, Y; Zhou, B; Zi, J (2013): Quantitative proteomics data using mTRAQ/MRM looking at human AKR family members in cancer cell lines GigaScience Database. http://dx.doi.org/10.5524/100047 RIS BibTeX Text

Members of the human aldo-keto reductase(AKR)superfamily have been reported to be involved in cancer progression, and to investigate their role further a quantitative method to measure human AKR proteins in cells using mTRAQ-based multiple reaction monitoring (MRM) has been developed. AKR peptides with multiple transitions were carefully selected upon tryptic digestion of the recombinant AKR proteins, while AKR proteins were identified by SDS-PAGE fractionation coupled with LC MS/MS. Utilizing mTRAQ triplex labeling to produce the derivative peptides, calibration curves were generated using the mixed lysate as background, and no significantly different quantification of AKRs was elicited from the two sets of calibration curves under the mixed and single lysate as background. This approach was employed to quantitatively determine the 6 AKR proteins, AKR1A1, AKR1B1, AKR1B10, AKR1C1/C2, AKR1C3 and AKR1C4 in 7 different cancer cell lines, and for the first time to obtain the absolute quantities of all the AKR proteins in each cell. The cluster plot revealed that AKR1A and AKR1B were widely distributed in most cancer cells with relatively stable abundances, whereas AKR1Cs were unevenly detected among cells demonstrating diverse dynamic abundances. The AKR quantitative distribution in different cancer cells, and may enable further insight on the role of AKR proteins are involved in tumorigenesis.

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Related manuscripts:

doi:10.1021/pr301153z (PubMed: 23544749)

Accessions (data included in GigaDB):

PeptideAtlas: PASS00087

Proteomic

http://gigadb.org/images/data/cropped/100047_MRM.png

Samples: Table Settings

Columns:

Common Name
Scienfic Name
Sample Attributes
Taxonomic ID
Genbank Name

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
56379606HumanhumanHomo sapiens Age:68
Disease status:grade II carcinoma
IHMC ethnicity:Caucasian
...
+
786-O9606HumanhumanHomo sapiens Age:58
Disease status:renal cell adenocarcinoma
IHMC ethnicity:Caucasian
...
+
A5499606HumanhumanHomo sapiens Age:58
Disease status:carcinoma
IHMC ethnicity:Caucasian
...
+
BGC8239606HumanhumanHomo sapiens Age:62
Disease status:adenocarcinoma
IHMC ethnicity:Chinese
...
+
H1579606HumanhumanHomo sapiens Age:84
Disease status:oral squamous cell carcinoma
Sex:male
...
+
HUH79606HumanhumanHomo sapiens Age:57
Disease status:hepato cellular carcinoma
IHMC ethnicity:Japanese
...
+
SW4809606HumanhumanHomo sapiens Disease status:adenocarcinoma grade III-IV
Tissue type:colon cell line
Displaying 1-7 of 7 Sample(s).

Files: (FTP site) Table Settings

Columns:

File Description
Sample ID
File Type
File Format
Size
Release Date
Download Link
File Attributes

File NameSample IDFile TypeFile FormatSizeRelease Date 
5637Multiple Reaction Monitoring (MRM)UNKNOWN2.32 MB2013-05-18
5637Multiple Reaction Monitoring (MRM)UNKNOWN453.5 KB2013-05-18
5637Multiple Reaction Monitoring (MRM)UNKNOWN2.32 MB2013-05-18
5637Multiple Reaction Monitoring (MRM)UNKNOWN454.07 KB2013-05-18
5637Multiple Reaction Monitoring (MRM)UNKNOWN2.32 MB2013-05-18
5637Multiple Reaction Monitoring (MRM)UNKNOWN454 KB2013-05-18
5637Multiple Reaction Monitoring (MRM)UNKNOWN2.32 MB2013-05-18
5637Multiple Reaction Monitoring (MRM)UNKNOWN453.33 KB2013-05-18
786-OMultiple Reaction Monitoring (MRM)UNKNOWN2.32 MB2013-05-18
786-OMultiple Reaction Monitoring (MRM)UNKNOWN455.06 KB2013-05-18
Displaying 1-10 of 176 File(s).

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