Macrotermes natalensis gut metagenome assembly data.

Dataset type: Metagenomic
Data released on September 01, 2014

Poulsen M; Hu H; Li C; Boomsma JJ; Zhang G (2014): Macrotermes natalensis gut metagenome assembly data. GigaScience Database. http://dx.doi.org/10.5524/100058

DOI10.5524/100058

Background: Most termites rely on gut microbial symbionts to decompose organic matter. During the process of Macrotermitinae domesticating Termitomyces fungi, it led to the loss of their ancestral gut symbionts and the acquisition a novel gut microbiome, but the complementary roles of the major components of this obligate symbiosis remain enigmatic. Here we provide sequence assemblies for the gut microbiome of 3 distinct castes within the a single colony of termite.
Sampling: The gut DNA were extract from major workers, minor soldiers and the queen from M. natalensis colony Mn117 (S24°40’30.5” E28°47’50.4”, elevation 1045 m). In workers and soldiers, duplicate extractions were performed on 2 x 50 guts pulled out of individuals aseptically, which were pooled after extraction. The queen gut DNA was from a entire queen gut dissected out aseptically.
Finding: The three gut microbiomes were assembled to 446Mb (>1,200,000 genes), 337Mb (>900,000 genes), and 33Mb (>50,000 genes) for workers, soldiers and the queen, respectively.

Additional details

Read the peer-reviewed publication(s):

Poulsen, M., Hu, H., Li, C., Chen, Z., Xu, L., Otani, S., … Zhang, G. (2014). Complementary symbiont contributions to plant decomposition in a fungus-farming termite. Proceedings of the National Academy of Sciences, 111(40), 14500–14505. doi:10.1073/pnas.1319718111

Related datasets:

doi:10.5524/100058 IsPartOf doi:10.5524/100055
doi:10.5524/100058 IsSupplementedBy doi:10.5524/100056
doi:10.5524/100058 IsSupplementedBy doi:10.5524/100057

Accessions (data generated as part of this study):

SRA: SRP020010





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
SAMN01991224433724termite gut metagenome termite gut metagenome Altitude:1045
Description:termite queen metagenome
Geographic location (country and/or sea,region):So...
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SAMN01991225433724termite gut metagenome termite gut metagenome Altitude:1045
Description:termite worker metagenome
Geographic location (country and/or sea,region):So...
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SAMN01991226433724termite gut metagenome termite gut metagenome Altitude:1045
Description:termite soldier metagenome
Geographic location (country and/or sea,region):So...
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Displaying 1-3 of 3 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
SAMPLE:SAMN01991224Sequence assemblyFASTA10.13 MB2013-07-15
SAMPLE:SAMN01991224AnnotationBED3.03 MB2013-07-15
SAMPLE:SAMN01991224Coding sequenceFASTA2.75 MB2013-07-15
SAMPLE:SAMN01991224Protein sequenceFASTA1.92 MB2013-07-15
SAMPLE:SAMN01991226Sequence assemblyFASTA90.61 MB2013-07-15
SAMPLE:SAMN01991226AnnotationBED55.85 MB2013-07-15
SAMPLE:SAMN01991226Coding sequenceFASTA66.35 MB2013-07-15
SAMPLE:SAMN01991226Protein sequenceFASTA46.24 MB2013-07-15
SAMPLE:SAMN01991225Sequence assemblyFASTA121.47 MB2013-07-15
SAMPLE:SAMN01991225AnnotationBED75.83 MB2013-07-15
Displaying 1-10 of 13 File(s).
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