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Data released on February 16, 2015

Supplemental materials for "Live time-lapse dataset of in vitro wound healing experiments."

Ben-Jacob, E; Kaplan, D; Natan, S; Tsarfaty, I; Zaritsky, A (2015): Supplemental materials for "Live time-lapse dataset of in vitro wound healing experiments." GigaScience Database. http://dx.doi.org/10.5524/100118 RIS BibTeX Text

The wound healing assay is the common method to study collective cell migration in-vitro. Computational analyses of live imaging exploit the rich temporal information and significantly improve understanding of complex phenomena that emerge during this mode of collective motility. Publicly available experimental data can allow application of new analyses to promote new discoveries, and assess algorithms’ capabilities to distinguish between different experimental conditions.
Here we present a freely-available dataset of 31 time-lapse in vitro wound healing experiments of two cell lines. It consists of 6 different experimental conditions with 4-6 replicates each, gathered to study the effects of a growth factor on collective cell migration. A full detailed description of the data, intermediately processed data, scripts and experimental validations (all available here) are reported in the GigaScience Datanote.
Links to "The Cell: an image library", found below, are to the raw image data; group 45451 contains the DA3 images, while group 44501 holds the MDCK images.

Contact Submitter

Related manuscripts:

doi:10.1186/s13742-015-0049-6

Additional information:

http://www.cellimagelibrary.org/groups/45451

http://www.cellimagelibrary.org/groups/44501

Accessions (data included in GigaDB):

GitHub: gigascience/paper-zaritsky2015

Imaging

/images/uploads/image_upload/Images_192.png

Samples: Table Settings

Columns:

Common Name
Scienfic Name
Sample Attributes
Taxonomic ID
Genbank Name

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
DA3_cell-line_Control10090Mousehouse mouseMus musculus Cell type:adenocarcinoma
Growth temperature:37
Passage number:15
...
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DA3_cell-line_+HGF/SF10090Mousehouse mouseMus musculus Cell type:adenocarcinoma
Growth temperature:37
Passage number:15
...
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DA3_cell-line_PHA10090Mousehouse mouseMus musculus Cell type:adenocarcinoma
Growth temperature:37
Passage number:15
...
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DA3_cell-line_PHA+HGF/SF10090Mousehouse mouseMus musculus Cell type:adenocarcinoma
Growth temperature:37
Passage number:15
...
+
MDCK_cell-line_Control9615dogsdogCanis lupus familiaris Cell type:epithelial
Growth temperature:37
Passage number:10
...
+
MDCK_cell-line_+HGF/SF9615dogsdogCanis lupus familiaris Cell type:epithelial
Growth temperature:37
Passage number:10
...
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Displaying 1-6 of 6 Sample(s).

Files: (FTP site) Table Settings

Columns:

File Description
Sample ID
File Type
File Format
Size
Release Date
Download Link
File Attributes

File NameSample IDFile TypeFile FormatSizeRelease Date 
Mixed archiveTAR11.1 GB2015-02-05
MDCK_cell-line_ControlImageLSM62.88 MB2015-01-22
MDCK_cell-line_ControlMixed archiveGZIP56.77 MB2015-01-22
MDCK_cell-line_ControlImageLSM62.88 MB2015-01-22
MDCK_cell-line_ControlMixed archiveGZIP58.28 MB2015-01-22
MDCK_cell-line_ControlImageLSM62.88 MB2015-01-22
MDCK_cell-line_ControlMixed archiveGZIP55.62 MB2015-01-22
MDCK_cell-line_+HGF/SFImageLSM62.88 MB2015-01-22
MDCK_cell-line_+HGF/SFMixed archiveGZIP56.1 MB2015-01-22
MDCK_cell-line_+HGF/SFImageLSM62.88 MB2015-01-22
Displaying 1-10 of 68 File(s).

History:

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