3-dimensional microCT reconstructions of brooding brittle stars

Dataset type: Imaging
Data released on September 24, 2015

Du Plessis A; Landschoff J; Griffiths CL (2015): 3-dimensional microCT reconstructions of brooding brittle stars GigaScience Database. http://dx.doi.org/10.5524/100130

DOI10.5524/100130

Brooding brittle stars have a special reproduction mode of retaining their eggs and juveniles inside respiratory body-sacs called bursae. In the past, studying this phenomenon required disturbance of the sample by dissecting the adult. This caused irreversible damage and made the sample unsuitable for future studies. Micro X-ray-computed tomography (µCT) is a promising technique, not only to visualise juveniles inside the bursae, but also retaining the sample intact and making the dataset of the scan available for future reference.
Seven µCT scans of five freshly fixed (70% ethanol) individuals, representing three differently-sized brittle star species, provided adequate image quality to determine the numbers, sizes and postures of internally-brooded young, as well as anatomy and morphology of adults. No staining agents were necessary to achieve high-resolution, high-contrast images, which permitted visualisations of both calcified and soft tissue. The raw data (projection, reconstruction images and an additional volume file for easy viewing) are hereby made publicly available for download.

Additional details

Read the peer-reviewed publication(s):

Landschoff, J., Du Plessis, A., & Griffiths, C. L. (2015). A dataset describing brooding in three species of South African brittle stars, comprising seven high-resolution, micro X-ray computed tomography scans. GigaScience, 4(1). doi:10.1186/s13742-015-0093-2





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
AmphCap_182377  Amphiura Collection date:40475
Common name:Equitailed brittle star
Geographic location (country and/or sea,region):So...
...
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AmphCap_282377  Amphiura Collection date:40475
Common name:Equitailed brittle star
Geographic location (country and/or sea,region):So...
...
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AmphSqua48271  Amphipholis squamata Collection date:40207
Common name:Scaly-armed brittle star
Geographic location (country and/or sea,region):So...
...
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OphWahl_172726  Ophioderma Collection date:40111
Common name:Serpent-skinned brittle star
Geographic location (country and/or sea,region):So...
...
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OphWahl_272726  Ophioderma Collection date:40111
Common name:Serpent-skinned brittle star
Geographic location (country and/or sea,region):So...
...
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OphWahl_372726  Ophioderma Collection date:40111
Common name:Serpent-skinned brittle star
Geographic location (country and/or sea,region):So...
...
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OphWahl_472726  Ophioderma Collection date:40111
Common name:Serpent-skinned brittle star
Geographic location (country and/or sea,region):So...
...
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Displaying 1-7 of 7 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
AmphCap_1VideoAVI2.38 MB2015-01-29
AmphCap_1MicroCTzip8.78 GB2015-01-29
AmphCap_1MicroCTzip4.92 GB2015-01-29
AmphCap_2MicroCTzip9.16 GB2015-01-29
AmphCap_2TextUNKNOWN2.82 KB2015-01-29
AmphCap_2MicroCTzip3.78 GB2015-01-29
AmphSquaVideoAVI32.97 MB2015-01-29
AmphSquaMicroCTzip11.67 GB2015-01-29
AmphSquaTextUNKNOWN2.8 KB2015-05-22
AmphSquaMicroCTzip2.07 GB2015-01-29
Displaying 1-10 of 23 File(s).
Date Action
September 25, 2015 Dataset publish
November 27, 2015 Manuscript Link added : 10.1186/s13742-015-0093-2