Data released on April 20, 2015
The brown planthopper, Nilaparvata lugens, the most destructive pest of rice, is a typical monophagous herbivore that feeds exclusively on rice sap, which migrates over long distances. Outbreaks of it have re-occurred approximately every three years in Asia. It has also been used as a model system for ecological studies and for developing effective pest management.
The N. lugens population sequenced in the present study was originally collected in Hangzhou, China, in 2008 and had been reared on rice seedlings (strain: TN1). One male and one female were isolated and mated to produce F1 progeny. A single pair was then selected for sibling inbreeding for 13 generations. Genomic DNA was extracted from adult females of the F13 generation. We constructed a series of DNA libraries with varying insert sizes (180 bp, 200 bp, 500 bp, 2 kb, 5 kb, 10 kb, 20 kb, and 40 kb) to perform WGS sequencing. All of the above libraries were sequenced on Illumina HiSeq 2000 or GA-II sequencers. After performing filtering steps to remove adapter-contaminated reads, low-quality reads, and duplicate reads, a total of 158.01 Gbp of clean WGS data (131.67X of the genome) with 131.7-fold of genome coverage depth and were combined with 507.94 Gbp of clean fosmid sequence data was obtained. This was assembled using SOAPdenovo and Rabbit, producing a final draft assembly of 1.141Gbp with a scaffold N50 of 356,597 bp. The Nilaparvata lugens genome should highlight potential directions for effective pest control of the planthopper.