Supporting scripts and data for "Investigation into the annotation of protocol sequencing steps in the Sequence Read Archive".

Dataset type: Metadata
Data released on May 11, 2015

Alnasir J; Shanahan H (2015): Supporting scripts and data for "Investigation into the annotation of protocol sequencing steps in the Sequence Read Archive". GigaScience Database. http://dx.doi.org/10.5524/100142

DOI10.5524/100142

The workflow for the production of high-throughput sequencing data from nucleic acid samples is complex. There are a series of protocol steps to be followed in the preparation of samples for next-generation sequencing. The quantification of bias in a number of protocol steps, namely DNA fractionation, blunting, phosphorylation, adapter ligation and library enrichment, remains to be determined. We examined the experimental metadata of the public repository Sequence Read Archive (SRA) in order to ascertain the level of annotation of important sequencing steps in submissions to the database.

Additional details

Read the peer-reviewed publication(s):

Alnasir, J., & Shanahan, H. P. (2015). Investigation into the annotation of protocol sequencing steps in the sequence read archive. GigaScience, 4(1). doi:10.1186/s13742-015-0064-7





File NameSample IDData TypeFile FormatSizeRelease Date 
ImageUNKNOWN54.25 KB2015-04-29
ImageUNKNOWN79.8 KB2015-04-30
ReadmeTEXT2.3 KB2015-04-29
OtherUNKNOWN366.11 MB2015-04-29
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Date Action
May 11, 2015 Dataset publish
May 11, 2015 Description updated from : The workflow for the production of high-throughput sequencing data from nucleic acid samples is complex. There are a series of protocol steps to be followed in the preparation of samples for next-generation sequencing. The quantification of bias in a number of protocol steps, namely DNA fractionation, blunting, phosphorylation, adapter ligation and library enrichment, remains to be determined. We examined the experimental metadata of the public repository Sequence Read Archive (SRA) in order to ascertain the level of annotation of important sequencing steps in submissions to the database.