Supporting data and materials for "Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant".

Dataset type: Metagenomic, Transcriptomic, Workflow, Virtual-Machine
Data released on July 28, 2015

Albersmeier A; Belmann P; Bremges A; Eikmeyer FG; Maus I; Pühler A; Schlüter A; Sczyrba A; Winkler A (2015): Supporting data and materials for "Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant". GigaScience Database. http://dx.doi.org/10.5524/100151

DOI10.5524/100151

The production of biogas takes place under anaerobic conditions and involves microbial decomposition of organic matter. Most of the participating microbes still have to be considered as unknown and non-cultivable. Accordingly, metagenome sequencing currently is the method of choice to obtain insights into community composition and the genetic repertoire. Here, we report the first deeply sequenced metagenome and metatranscriptome of a complex biogas-producing microbial community from an agricultural production-scale biogas plant. We assembled the metagenome and reconstructed most genes involved in the methane metabolism, a key pathway involving methanogenesis performed by methanogenic Archaea. This exemplary result indicates sufficient sequencing coverage for most downstream analyses. Sequenced at least one order of magnitude deeper than previous studies, our metagenome data will enable novel insights into community composition and the genetic potential of important community members. Moreover, mapping of transcripts to reconstructed genome sequences will enable the identification of active metabolic pathways in target organisms.

Additional details

Read the peer-reviewed publication(s):

Bremges, A., Maus, I., Belmann, P., Eikmeyer, F., Winkler, A., Albersmeier, A., … Sczyrba, A. (2015). Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant. GigaScience, 4(1). doi:10.1186/s13742-015-0073-6

Additional information:

https://registry.hub.docker.com/u/metagenomics/2015-biogas-cebitec/

https://github.com/metagenomics/2015-biogas-cebitec

Accessions (data included in GigaDB):

BioProject: PRJEB8813





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
Biogas_Metagenome_1718289biogas fermenter metagenomemetagenomesbiogas fermenter metagenome Reaction vessel volume:2041
Reaction vessel height:6.4
Reaction vessel diameter:21
...
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Biogas_Metagenome_2718289biogas fermenter metagenomemetagenomesbiogas fermenter metagenome Reaction vessel volume:2041
Reaction vessel height:6.4
Reaction vessel diameter:21
...
+
Biogas_Metagenome_3718289biogas fermenter metagenomemetagenomesbiogas fermenter metagenome Reaction vessel volume:2041
Reaction vessel height:6.4
Reaction vessel diameter:21
...
+
Biogas_Metagenome_4718289biogas fermenter metagenomemetagenomesbiogas fermenter metagenome Reaction vessel volume:2041
Reaction vessel height:6.4
Reaction vessel diameter:21
...
+
Biogas_Metagenome_5718289biogas fermenter metagenomemetagenomesbiogas fermenter metagenome Reaction vessel volume:2041
Reaction vessel height:6.4
Reaction vessel diameter:21
...
+
Biogas_Metagenome_6718289biogas fermenter metagenomemetagenomesbiogas fermenter metagenome Reaction vessel volume:2041
Reaction vessel height:6.4
Reaction vessel diameter:21
...
+
Biogas_Metatranscriptome718289biogas fermenter metagenomemetagenomesbiogas fermenter metagenome Reaction vessel volume:2041
Reaction vessel height:6.4
Reaction vessel diameter:21
...
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Displaying 1-7 of 7 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
Otherzip216 MB2015-07-22
Sequence assemblyFASTA68.35 MB2015-07-22
OtherKEGG5.03 MB2015-07-22
OtherKEGG6.38 MB2015-07-22
OtherFASTA44.96 MB2015-07-22
Coding sequenceFASTA66.33 MB2015-07-22
OtherGFF11 MB2015-07-22
AnnotationGFF10.44 MB2015-07-22
OtherBAM6.9 GB2015-07-22
OtherBAM4.14 GB2015-07-22
Displaying 1-10 of 16 File(s).
Date Action
July 29, 2015 Dataset publish
August 24, 2015 Dataset Type added : Virtual Machine
November 2, 2015 File Contigs.prodigal.faa.blastp.kegg.annotated.tsv.gz updated