Supporting data and materials for "Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant".
Dataset type: Metagenomic, Virtual-Machine, Transcriptomic, Workflow
Data released on July 28, 2015
Bremges A; Maus I; Belmann P; Eikmeyer FG; Winkler A; Albersmeier A; Pühler A; Schlüter A; Sczyrba A (2015): Supporting data and materials for "Deeply sequenced metagenome and metatranscriptome of a biogas-producing microbial community from an agricultural production-scale biogas plant". GigaScience Database. http://dx.doi.org/10.5524/100151
The production of biogas takes place under anaerobic conditions and involves microbial decomposition of organic matter. Most of the participating microbes still have to be considered as unknown and non-cultivable. Accordingly, metagenome sequencing currently is the method of choice to obtain insights into community composition and the genetic repertoire.
Here, we report the first deeply sequenced metagenome and metatranscriptome of a complex biogas-producing microbial community from an agricultural production-scale biogas plant. We assembled the metagenome and reconstructed most genes involved in the methane metabolism, a key pathway involving methanogenesis performed by methanogenic Archaea. This exemplary result indicates sufficient sequencing coverage for most downstream analyses.
Sequenced at least one order of magnitude deeper than previous studies, our metagenome data will enable novel insights into community composition and the genetic potential of important community members. Moreover, mapping of transcripts to reconstructed genome sequences will enable the identification of active metabolic pathways in target organisms.
Additional details
Read the peer-reviewed publication(s):
Additional information:
https://registry.hub.docker.com/u/metagenomics/2015-biogas-cebitec/
https://github.com/metagenomics/2015-biogas-cebitec
Accessions (data generated as part of this study):
BioProject:
PRJEB8813
Sample ID | Taxonomic ID | Common Name | Genbank Name | Scientific Name | Sample Attributes |
---|---|---|---|---|---|
Biogas_Metagenome_1 | 718289 | biogas fermenter metagenome | metagenomes | biogas fermenter metagenome | Reaction vessel volume:2041 Reaction vessel height:6.4 Reaction vessel diameter:21 ... + |
Biogas_Metagenome_2 | 718289 | biogas fermenter metagenome | metagenomes | biogas fermenter metagenome | Reaction vessel volume:2041 Reaction vessel height:6.4 Reaction vessel diameter:21 ... + |
Biogas_Metagenome_3 | 718289 | biogas fermenter metagenome | metagenomes | biogas fermenter metagenome | Reaction vessel volume:2041 Reaction vessel height:6.4 Reaction vessel diameter:21 ... + |
Biogas_Metagenome_4 | 718289 | biogas fermenter metagenome | metagenomes | biogas fermenter metagenome | Reaction vessel volume:2041 Reaction vessel height:6.4 Reaction vessel diameter:21 ... + |
Biogas_Metagenome_5 | 718289 | biogas fermenter metagenome | metagenomes | biogas fermenter metagenome | Reaction vessel volume:2041 Reaction vessel height:6.4 Reaction vessel diameter:21 ... + |
Biogas_Metagenome_6 | 718289 | biogas fermenter metagenome | metagenomes | biogas fermenter metagenome | Reaction vessel volume:2041 Reaction vessel height:6.4 Reaction vessel diameter:21 ... + |
Biogas_Metatranscriptome | 718289 | biogas fermenter metagenome | metagenomes | biogas fermenter metagenome | Reaction vessel volume:2041 Reaction vessel height:6.4 Reaction vessel diameter:21 ... + |