Supporting material for "High throughput identification of novel conotoxins from the Chinese tubular cone snail (Conus betulinus) by multi-transcriptome sequencing".

Dataset type: Transcriptomic
Data released on April 07, 2016

Peng C; Yao G; Gao B; Fan C; Bian C; Wang J; Cao Y; Wen B; Zhu Y; Ruan Z; Zhao X; You X; Bai J; Li J; Lin Z; Zou S; Zhang X; Qiu Y; Chen J; Coon SL; Yang J; Chen J; Shi Q (2016): Supporting material for "High throughput identification of novel conotoxins from the Chinese tubular cone snail (Conus betulinus) by multi-transcriptome sequencing". GigaScience Database.


The venom of predatory marine cone snails mainly contains a diverse array of unique bioactive peptides commonly referred to as conopeptides or conotoxins. These peptides have proven to be valuable pharmacological probes and potential drugs because of their high specificity and affinity to important ion channels, receptors and transporters of the nervous system. Most of previous studies have specifically focused on the conopeptides from piscivorous and molluscivorous cone snails, but little attention has been devoted to the dominant vermivorous species. The vermivorous Chinese tubular cone snail, Conus betulinus, is the dominant Conus species inhabiting in the South China Sea. The transcriptomes of venom ducts and venom bulbs from six specimens of this species were sequenced using both next-generation sequencing and traditional Sanger sequencing technologies, resulting in the identification of a total of 215 distinct conopetides. Among these, 183 were novel conopeptides, including 9 new superfamilies. It appears that most of the identified conopeptides were synthesized in the venom duct, while a handful of conopeptides were identified only in the venom bulb at very low levels. A variance in conopeptides from different specimens of C. betulinus was observed, which suggested presence of intraspecific variability in toxicity at the genetic level. The raw sequence data are available from the Sequence Read Archives, and here we provide the BLAST resutls and our annotations of this potentially fertile resource for the development of new pharmaceutics.

Additional details

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Accessions (data generated as part of this study):

BioProject: PRJNA290540

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
Big89764  Conus betulinus Height or length:10
Tissue:venom duct
Specimen voucher:1
Bulb89764  Conus betulinus Height or length:8.7
Tissue:venom bulb
Specimen voucher:2
EST89764  Conus betulinus Height or length:various
Tissue:venom ducts
Specimen voucher:1,3,5,6,7,& 8
Middle89764  Conus betulinus Height or length:8.7
Tissue:venom duct
Specimen voucher:2
Normalized89764  Conus betulinus Height or length:8.5
Tissue:venom duct
Specimen voucher:4
Small89764  Conus betulinus Height or length:6
Tissue:venom duct
Specimen voucher:3
Displaying 1-6 of 6 Sample(s).

File NameSample IDData TypeFile FormatSizeRelease Date 
BigProtein sequencearchive6.59 KB2016-04-06
BigOtherarchive139.24 KB2016-04-06
BulbProtein sequencearchive4.95 KB2016-04-06
BulbOtherarchive127.11 KB2016-04-06
NormalizedAnnotationEXCEL15.86 MB2016-04-06
NormalizedBLASTUNKNOWN95.95 KB2016-04-06
NormalizedProtein sequenceFASTA5.57 MB2016-04-06
NormalizedCoding sequenceFASTA15.65 MB2016-04-06
BigAnnotationEXCEL13.84 MB2016-04-06
BigBLASTUNKNOWN11.7 KB2016-04-06
Displaying 1-10 of 38 File(s).
Funding body Awardee Award ID Comments
China 863 Project 2014AA093501
Education and Research of Guangdong Province 2013B090800017
Shenzhen Scientific Research and Development CXB201108250095A
Date Action
April 7, 2016 Dataset publish
April 15, 2016 Manuscript Link added : 10.1186/s13742-016-0122-9