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Data released on May 26, 2016

Supporting data for "Sequencing, de novo assembly, and annotation of a pink bollworm larval midgut transcriptome".

Carriere, Y; Fabrick, J, A; Flexner, J, L; Mathis, J; Nelson, M, E; Tabashnik, B, E; Tassone, E, E; Wu, G; Zastrow-Hayes, G (2016): Supporting data for "Sequencing, de novo assembly, and annotation of a pink bollworm larval midgut transcriptome". GigaScience Database. http://dx.doi.org/10.5524/100203 RIS BibTeX Text

The pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) is one of the world's most important pests of cotton. Insecticide sprays and transgenic cotton producing toxins of the bacterium Bacillus thuringiensis (Bt) are currently used to manage this pest. Bt toxins kill susceptible insects by specifically binding to and destroying midgut cells, but they are not toxic to most other organisms. Pink bollworm is useful as a model for understanding insect responses to Bt toxins, yet advances in understanding at the molecular level have been limited because basic genomic information is lacking for this cosmopolitan pest. Here, we have sequenced, de novo assembled and annotated a comprehensive larval midgut transcriptome from a susceptible strain of pink bollworm.
A de novo transcriptome assembly for the midgut of P. gossypiella was generated containing 46,458 transcripts (average length of 770 bp) derived from 39,874 unigenes. The size of the transcriptome is similar to published midgut transcriptomes of other Lepidoptera and includes up to 91% annotated contigs.
Foundational knowledge of protein-coding genes from the pink bollworm midgut is critical for understanding how this important insect pest functions. The transcriptome data presented here represents the first large-scale molecular resource for this species, and may be used for deciphering relevant midgut proteins critical for xenobiotic detoxification, nutrient digestion and allocation, as well as for the discovery of protein receptors important for Bt intoxication.

Contact Submitter

Related manuscripts:

doi:10.1186/s13742-016-0130-9

Accessions (data included in GigaDB):

BioProject: PRJNA293973
SRA: GDQN00000000

Keywords:

transcriptome midgut pink bollworm Pectinophora gossypiella Bacillus thuringiensis 

Transcriptomic

http://gigadb.org/images/data/cropped/100203.jpg

Funding:

  • Funding body -
  • Comment - This is a cooperative investigation between USDA ARS the University of Arizona and DuPont-Pioneer
  • Funding body - DuPont
  • Award ID - DuPont-Pioneer agreement #58-3K95-4-1666
  • Comment - Jeffrey A Fabrick
  • Funding body - DuPont
  • Award ID - DuPont-Pioneer agreement #58-3K95-4-1666
  • Comment - Bruce E Tabashnik
  • Funding body - DuPont
  • Award ID - DuPont-Pioneer agreement #58-3K95-4-1666
  • Comment - Yves Carriere

Samples: Table Settings

Columns:

Common Name
Scienfic Name
Sample Attributes
Taxonomic ID
Genbank Name

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
SAMN0401268713191 pink bollwormPectinophora gossypiella Common name:pink bollworm
Strain:APHIS-S
Description:APHIS-S is a Bacillus thuringiensis (B...
...
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SAMN0401268813191 pink bollwormPectinophora gossypiella Common name:pink bollworm
Strain:APHIS-S
Description:APHIS-S is a Bacillus thuringiensis (B...
...
+
SAMN0401268913191 pink bollwormPectinophora gossypiella Common name:pink bollworm
Strain:APHIS-S
Description:APHIS-S is a Bacillus thuringiensis (B...
...
+
Displaying 1-3 of 3 Sample(s).

Files: (FTP site) Table Settings

Columns:

File Description
Sample ID
File Type
File Format
Size
Release Date
Download Link
File Attributes

File NameSample IDFile TypeFile FormatSizeRelease Date 
AnnotationIPR21.95 MB2016-05-12
sequence assemblyFASTA19.27 MB2016-05-12
BLASTTSV3.6 MB2016-05-12
Tabular dataCSV2.28 MB2016-05-12
BLASTTSV2.59 MB2016-05-12
sequence assemblyFASTA42.1 MB2016-05-12
ReadmeTEXT1.44 KB2016-05-12
TXTUNKNOWN0.36 KB2016-05-12
BLASTXML3.84 GB2016-05-16
AnnotationGFF16.67 MB2016-05-12
Displaying 1-10 of 10 File(s).

History:

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