Data and analysis of diet-induced and obesity-associated alterations of gut microbiota of 129S6/Sv and C57BL/6J mice

Dataset type: Metagenomic
Data released on January 25, 2017

Xiao L; Sonne SB; Feng Q; Chen N; Xia Z; Li X; Fang Z; Zhang D; Fjære E; Midtbø LK; Derrien M; Hugenholtz F; Li J; Zhang J; Liu C; Hao Q; Vogel UB; Mortensen A; UR Kleerebezem MW; Licht TR; Li Y; Arumugam M; Wang J; Madsen L; Kristiansen K (2017): Data and analysis of diet-induced and obesity-associated alterations of gut microbiota of 129S6/Sv and C57BL/6J mice GigaScience Database. http://dx.doi.org/10.5524/100271

DOI10.5524/100271

It is well known that the microbiota of high fat (HF) diet-induced obese mice differs from that of lean mice, but to what extent this difference reflects the obese state or the diet is unclear. To dissociate changes in the gut microbiota associated with high HF feeding from those associated with obesity, we took advantage of the different susceptibility of C57BL/6JBomTac (BL6) and 129S6/SvEvTac (Sv129) mice to diet-induced obesity and of their different responses to inhibition of cyclooxygenase (COX) activity, where inhibition of COX activity in BL6 mice prevents HF diet-induced obesity, but in Sv129 mice accentuates obesity.
Using HiSeq-based whole genome sequencing we identified taxonomic and functional differences in the gut microbiota of the two mouse strains fed regular low fat or HF diets with or without supplementation with the COX-inhibitor, indomethacin.
Here we present the sequence assemblies and annotations for those 54 samples, together with the gene catalogue and relevative abundance levels of both genes and OTUs. It is hoped these data can be used for comparison in future studies of a similar design.

Additional details

Read the peer-reviewed publication(s):

(PubMed: 28390422)

Accessions (data generated as part of this study):

ENA: ERP011540





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
indo_1410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811228
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_10410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811237
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_11410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811238
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_12410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811239
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_13410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811240
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_14410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811241
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_15410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811242
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_16410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811243
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_17410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811244
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
indo_18410661mouse gut metagenomemouse gut metagenome Alternative accession-SRA Sample:ERS811245
Body product:feces [ENVO:00002003]
Environment (feature):anthropogenic environment [E...
...
+
Displaying 1-10 of 54 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
indo_10Sequence assemblyFASTA25.14 MB2017-01-10
indo_11Sequence assemblyFASTA35.91 MB2017-01-10
indo_12Sequence assemblyFASTA32.93 MB2017-01-10
indo_13Sequence assemblyFASTA24.24 MB2017-01-10
indo_14Sequence assemblyFASTA32.03 MB2017-01-10
indo_15Sequence assemblyFASTA37.56 MB2017-01-10
indo_16Sequence assemblyFASTA31.05 MB2017-01-10
indo_17Sequence assemblyFASTA37.8 MB2017-01-10
indo_18Sequence assemblyFASTA40.91 MB2017-01-10
indo_19Sequence assemblyFASTA24.34 MB2017-01-10
Displaying 1-10 of 71 File(s).
Funding body Awardee Award ID Comments
Danish National Research Foundation
Carlsbergfondet
National Institute Of Nutrition And Seafood Research
Danish Strategic Research Council 11-116163
Shenzhen Municipal Government of China CXZZ20150330171521403
Shenzhen Municipal Government of China JCYJ20140418095735538
Date Action
January 25, 2017 Dataset publish
November 13, 2017 Manuscript Link added : 10.1186/s40168-017-0258-6