Draft genomes of two blister beetles Hycleus cichorii and Hycleus phaleratus

Dataset type: Genomic
Data released on February 05, 2018

Wu Y; Li J; Chen X (2018): Draft genomes of two blister beetles Hycleus cichorii and Hycleus phaleratus GigaScience Database. http://dx.doi.org/10.5524/100405

DOI10.5524/100405

Commonly known as blister beetles or Spanish fly, there are more than 1,500 species in the Meloidae family (Hexapoda:Coleoptera:Tenebrionoidea) that produce the potent defensive blistering agent cantharidin. Cantharidin and its derivatives have been used to treat cancers, such as liver, stomach, lung and esophageal cancers. Hycleus cichorii and Hycleus phaleratus are the most commercially important blister beetles due to their ability to biosynthesize this potent vesicant. However, there is a lack of genome reference, which has hindered development of studies on the biosynthesis of cantharidin and a better understanding of its biology and pharmacology. We report two draft genomes and quantified gene sets for blister beetles H. cichorii and H. phaleratus, two complex genomes with >72 % repeats and ~1 % heterozygosity, using Illumina sequencing data. An integrated assembly pipeline was performed for assembly and most of the coding regions were obtained. BUSCO assessment showed that our assembly obtained more than 98 % of the Endopterygota universal single-copy orthologs. Comparison analysis showed that the completeness of coding gene in our assembly was comparable to other beetle genomes such as Dendroctonus ponderosae and Agrilus planipennis. Gene annotation yielded 13,813 and 13,725 protein-coding genes in H. cichorii and H. phaleratus, of which ~89 % were functionally annotated. BUSCO assessment showed that ~86 and 84 % of the Endopterygota universal single-copy orthologs were annotated completely in these two gene sets, whose completeness is comparable to that of D. ponderosae and A. planipennis. Assembly of the both blister beetle genomes provide a valuable resource for future biosynthesis of cantharidin and comparative genomic studies of blister beetles and other beetles.

Additional details

Read the peer-reviewed publication(s):

Wu, Y.-M., Li, J., & Chen, X.-S. (2018). Draft genomes of two blister beetles Hycleus cichorii and Hycleus phaleratus. GigaScience, 7(3). doi:10.1093/gigascience/giy006

Accessions (data included in GigaDB):

BioProject: PRJNA390850

Accessions (data not in GigaDB):

BioProject: PRJNA349771
BioProject: PRJNA381455





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
SAMN072515931270216  Hycleus cichorii Alternative accession-BioProject:PRJNA390850
Description:male of Hycleus cichorii
Sex:male
...
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SAMN072515941248972  Hycleus phaleratus Alternative accession-BioProject:PRJNA390850
Description:male of Hycleus phaleratus
Sex:male
...
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Displaying 1-2 of 2 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
BLASTUNKNOWN73.93 MB2018-01-25
annotationTAR1.36 KB2018-01-25
Coding SequenceFASTA4.98 MB2018-01-25
annotationEXCEL1.35 MB2018-01-25
Sequence assemblyFASTA30.8 MB2018-01-25
annotationGFF673.36 KB2018-01-25
protein sequenceFASTA3.39 MB2018-01-25
Repeat sequenceGFF4.78 MB2018-01-25
Coding SequenceFASTA4.86 MB2018-01-25
annotationEXCEL1.42 MB2018-01-25
Displaying 1-10 of 16 File(s).
Funding body Awardee Award ID Comments
National Natural Science Foundation of China Xiang-Sheng Chen 81460576 Morphological and molecular identification of medical meloid species and evaluation on diversity of cantharidin resources in Southwest Region
Science and Technology Foundation of Guizhou Province Xiang-Sheng Chen 20144001 Science and Technology Innovation Talents Team for Development and Utilization of Arthropod Resources, Guizhou Province
Science and Technology Foundation of Guizhou Province Xiang-Sheng Chen 20154021 Excellent Innovation Talents (Hundred Level), Guizhou Province
Science and Technology Foundation of Guizhou Province Xiang-Sheng Chen 20165802 International Cooperation Base for Insect Evolutionary Biology and Pest Control
Date Action
February 5, 2018 Dataset publish
July 3, 2018 Manuscript Link added : 10.1093/gigascience/giy006