The PE150 reference human genome dataset of the BGISEQ-500 and NovaSeq6000 sequencer

Dataset type: Genomic
Data released on April 30, 2018

Huang J; Liang X; Xuan Y; Geng C; Li Y; Lu H; Qu S; Mei X; Che H; Yu T; Sun N; Rao J; Wang J; Ying Chen WZ; Liao S; Jiang H; Liu X; Yang Z; Mu F; Gao S (2018): The PE150 reference human genome dataset of the BGISEQ-500 and NovaSeq6000 sequencer GigaScience Database. http://dx.doi.org/10.5524/100449

DOI10.5524/100449

The BGISEQ-500 is a new desktop sequencer developed by BGI. Using DNA nanoballs (DNB) and combinational probe-anchor synthesis (cPAS) developed from Complete Genomics sequencing technology, it generates short reads at a large scale, which can help fulfill the growing demands for sequencing. Here, we present the first human whole genome sequencing dataset from the BGISEQ-500. The dataset was generated by sequencing the widely-used Genome in a Bottle Consortium cell line, HG001 (NA12878). We have previously released the paired end 50bp (PE50) sequences (DOI:10.5524/100252) and paired end (PE100) sequences (DOI:10.5524/100274). Now we present the PE150 reads from same sample and library protocol, together with the PE150 PCR library of Illumina new sequencer. We also included the base and quality distribution figures of the new datasets. Moreover, we refined the variation calling parameters based on the new and previously dataset and compared that to similar amounts of publicly available NovaSeq6000 data from Illumina BaseSpace Sequence Hub.

Keywords:

Additional details

Read the peer-reviewed publication(s):

Huang, J., Liang, X., Xuan, Y., Geng, C., Li, Y., Lu, H., … Gao, S. (2017). A reference human genome dataset of the BGISEQ-500 sequencer. GigaScience, 6(5). doi:10.1093/gigascience/gix024

Related datasets:

doi:10.5524/100449 IsNewVersionOf doi:10.5524/100274

Accessions (data generated as part of this study):

BioProject: PRJEB25641





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
BGISEQ-500_PE150-19606HumanhumanHomo sapiens Description:Genome in A Bottle Consortium cell lin...
Alternative accession-BioProject:PRJEB25641
Alternative accession-BioSample:SAMEA104707357
...
+
BGISEQ-500_PE150-29606HumanhumanHomo sapiens Description:Genome in A Bottle Consortium cell lin...
Alternative accession-BioProject:PRJEB25641
Alternative accession-BioSample:SAMEA104707358
...
+
NovaSeq6000_Basespace_PE1509606HumanhumanHomo sapiens Description:Genome in A Bottle Consortium cell lin...
Alternative accession-BioProject:PRJEB25641
Alternative accession-BioSample:SAMEA104707359
...
+
Displaying 1-3 of 3 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
BGISEQ-500_PE150-1ImagePNG21.66 KB2018-04-30
BGISEQ-500_PE150-1ImagePNG15.33 KB2018-04-30
BGISEQ-500_PE150-1Sequence variantsVCF181.83 MB2018-04-30
BGISEQ-500_PE150-1OtherVCF1.56 MB2018-04-30
BGISEQ-500_PE150-2ImagePNG21.47 KB2018-04-30
BGISEQ-500_PE150-2ImagePNG15.47 KB2018-04-30
BGISEQ-500_PE150-2Sequence variantsVCF181.6 MB2018-04-30
BGISEQ-500_PE150-2OtherVCF1.56 MB2018-04-30
ArticleUNKNOWN19.68 KB2018-04-30
TextTEXT0.74 KB2018-04-30
Displaying 1-10 of 19 File(s).
Date Action
July 3, 2018 Dataset publish
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-1
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-1
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-1
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-1
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-1
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-1
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-1
July 7, 2018 Sample Attribute added : of Sample NovaSeq6000_Basespace_PE150
July 7, 2018 Sample Attribute added : of Sample NovaSeq6000_Basespace_PE150
July 7, 2018 Sample Attribute added : of Sample NovaSeq6000_Basespace_PE150
July 7, 2018 Sample Attribute added : of Sample NovaSeq6000_Basespace_PE150
July 7, 2018 Sample Attribute added : of Sample NovaSeq6000_Basespace_PE150
July 7, 2018 Sample Attribute added : of Sample NovaSeq6000_Basespace_PE150
July 7, 2018 Sample Attribute added : of Sample NovaSeq6000_Basespace_PE150
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-2
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-2
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-2
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-2
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-2
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-2
July 7, 2018 Sample Attribute added : of Sample BGISEQ-500_PE150-2