Supporting data for "Metabolomics Investigation of Dietary Effects on Flesh Quality in Grass Carp (Ctenopharyngodon idellus)"

Dataset type: Metabolomic
Data released on August 23, 2018

Zhao H; Chong J; Tang R; Li L; Xia J; Li D (2018): Supporting data for "Metabolomics Investigation of Dietary Effects on Flesh Quality in Grass Carp (Ctenopharyngodon idellus)" GigaScience Database. http://dx.doi.org/10.5524/100494

DOI10.5524/100494

The ultra-high density intensive farming model of grass carp (Ctenopharyngodon idellus) may elicit growth inhibition, decline flesh quality and increase disease susceptibility of fish. The quality degradation and excessive fat accumulation in cultured C. idellus have long been attributed to possible alterations in the lipid metabolism of fish muscle tissues as a result of over-nutrition from artificial diets. To investigate the effects of different diets on fish muscle quality, a large-scale metabolomics study was performed on 250 tails of C. idellus
. The experimental fish were divided into four groups based on sex and diets - female artificial feed (FAF), female grass feed (FGF), male artificial feed (MAF) and male grass feed (MGF). After 113 days rearing period, the AF group showed significantly higher total mass of muscle fat (P < 0.01), with the FAF group being the highest. Metabolomics profiling based on liquid chromatography-mass spectrometry (LC-MS) revealed distinctive patterns of clustering according to the four groups. Overall, artificial feeding was associated with higher concentrations of docosapentaenoic acid (DPA), dihomo-gamma-linolenic acid (DGLA) and arachidonic acid (ARA); whereas grass-feeding was associated with elevated n-3 unsaturated fatty acids (n-3 UFAs), such as eicosapentaenoic acid (EPA), alpha-linolenic acid (ALA) and gamma-linolenic acid (GLA). Artificial feeding also resulted in significant increased docosahexaenoic acid (DHA) in MAF muscle than MGF fish, whereas no significance in the comparison of female samples. Metabolic pathway analyses using both targeted and untargeted approaches consistently revealed that the arachidonic acid metabolism and steroid hormone biosynthesis pathways were significantly different between AF and GF groups.
Our results suggested that grass is a better source of diet fatty acid and protein when compared to artificial feed. Grass feeding could effectively lower triglycerides in serum, reduce fat accumulation and alter lipid compositions in fish muscle by increasing the concentrations of n-3 UFAs, leading to better nutrition and health.

Additional details

Read the peer-reviewed publication(s):

Zhao, H., Chong, J., Tang, R., Li, L., Xia, J., & Li, D. (2018). Metabolomics investigation of dietary effects on flesh quality in grass carp (Ctenopharyngodon idellus). GigaScience, 7(10). doi:10.1093/gigascience/giy111

Additional information:

https://github.com/zhao253091640/HZAU-Prof.-Dapeng-Li-s-Laboratory

Accessions (data included in GigaDB):

MTBLS: MTBLS673





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
AF_107959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
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AF_127959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
+
AF_147959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
+
AF_177959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
+
AF_197959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
+
AF_217959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
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AF_237959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
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AF_257959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
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AF_67959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
+
AF_87959 grass carpCtenopharyngodon idella Description:The metabonomics test sample was colle...
Experiment type:LC-MS
Geographic location (country and/or sea,region):Ch...
...
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Displaying 1-10 of 236 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
AF-F-104imageUNKNOWN9 MB2018-08-17
AF-F-11imageUNKNOWN9 MB2018-08-17
AF-F-144imageUNKNOWN9 MB2018-08-17
AF-F-145imageUNKNOWN9 MB2018-08-17
AF-F-163imageUNKNOWN9 MB2018-08-17
AF-F-22imageUNKNOWN9 MB2018-08-17
AF-F-35imageUNKNOWN9 MB2018-08-17
AF-F-3imageUNKNOWN9 MB2018-08-17
AF-F-40imageUNKNOWN9 MB2018-08-17
AF-F-52imageUNKNOWN9 MB2018-08-17
Displaying 1-10 of 180 File(s).
Funding body Awardee Award ID Comments
China Agriculture Research System D Li CARS-45
National Natural Science Foundation of China D Li 31502140
Fundamental Research Funds for the Central Universities D Li 2662015PY119
China Scholarship Council H Zhao 201706760039

Protocols.io:

Date Action
August 23, 2018 Dataset publish
August 28, 2018 Manuscript Link added : 10.1093/gigascience/giy111