Supporting data for "Exploring the genome and transcriptome of the cave nectar bat Eonycteris spelaea with PacBio long-read sequencing"

Dataset type: Genomic, Transcriptomic
Data released on September 06, 2018

Wen M; J Ng JH; Zhu F; Chionh YT; Chia WN; Mendenhall IH; Y-H Lee BP; Irving AT; Wang L (2018): Supporting data for "Exploring the genome and transcriptome of the cave nectar bat Eonycteris spelaea with PacBio long-read sequencing" GigaScience Database. http://dx.doi.org/10.5524/100500

DOI10.5524/100500

In the past two decades, bats have emerged as an important model system to study host-pathogen interactions. More recently, it has been shown that bats may also serve as a new and excellent model to study aging, inflammation and cancer among other important biological processes. The cave nectar bat or lesser dawn bat (Eonycteris spelaea), is known to be a reservoir for several viruses and intracellular bacteria. It is a widely-distributed bat species throughout the tropics and subtropics from India to Southeast Asia, and pollinates several plant species, including the culturally and economically important durian in the region. Here, we report the whole-genome and transcriptome sequencing, followed by subsequent de novo assembly of the E. spelaea genome solely using the PacBio® long-read sequencing platform.
The newly assembled E. spelaea genome is 1.97 Gb in length and consists of 4,470 sequences with a contig N50 of 8.0 Mb. Identified repeat elements covered 34.65% of the genome and 20,640 unique protein coding genes with 39,526 transcripts were annotated.
We demonstrated that PacBio long-read sequencing platform alone is sufficient to generate a comprehensive de novo assembled genome and transcriptome of an important bat species. These results will provide useful insights and act as a resource to expand our understanding of bat evolution, ecology, physiology, immunology, viral infection and transmission dynamics.

Additional details

Read the peer-reviewed publication(s):

Wen, M., Ng, J. H. J., Zhu, F., Chionh, Y. T., Chia, W. N., Mendenhall, I. H., … Wang, L.-F. (2018). Exploring the genome and transcriptome of the cave nectar bat Eonycteris spelaea with PacBio long-read sequencing. GigaScience, 7(10). doi:10.1093/gigascience/giy116

Accessions (data included in GigaDB):

BioProject: PRJNA427241
SRA : SRP136892





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
SAMN0879438158065 lesser dawn batEonycteris spelaea Description:Pooled whole body RNA extracted from a...
Analyte type:RNA
Geographic location (latitude and longitude):1.341...
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SAMN0879438258065 lesser dawn batEonycteris spelaea Description:Pooled whole body RNA extracted from a...
Analyte type:RNA
Geographic location (latitude and longitude):1.341...
...
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SAMN0889753258065 lesser dawn batEonycteris spelaea Description:DNA extracted from Kidney of a male le...
Analyte type:DNA
Geographic location (latitude and longitude):1.341...
...
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SAMN0889778458065 lesser dawn batEonycteris spelaea Description:DNA extracted from lung of a male less...
Analyte type:DNA
Geographic location (latitude and longitude):1.341...
...
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Displaying 1-4 of 4 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
scriptPython5.26 KB2018-09-03
AlignmentsUNKNOWN145.46 KB2018-09-03
AlignmentsUNKNOWN23.25 KB2018-09-03
AlignmentsUNKNOWN128.55 KB2018-09-03
AlignmentsUNKNOWN66.26 KB2018-09-03
AlignmentsUNKNOWN144.32 KB2018-09-03
AlignmentsUNKNOWN120.8 KB2018-09-03
AlignmentsUNKNOWN59.09 KB2018-09-03
AlignmentsUNKNOWN58.67 KB2018-09-03
AlignmentsUNKNOWN46.95 KB2018-09-03
Displaying 1-10 of 1236 File(s).
Date Action
September 6, 2018 Dataset publish
November 3, 2018 Manuscript Link added : 10.1093/gigascience/giy116