Supporting data for "Genomes of trombidid mites reveal novel predicted allergens and laterally transferred genes associated with secondary metabolism"

Dataset type: Genomic, Proteomic
Data released on October 15, 2018

Dong X; Chaisiri K; Xia D; Armstrong SD; Fang Y; Donnelly MJ; Kadowaki T; McGarry JW; Darby AC; Makepeace BL (2018): Supporting data for "Genomes of trombidid mites reveal novel predicted allergens and laterally transferred genes associated with secondary metabolism" GigaScience Database. http://dx.doi.org/10.5524/100505

DOI10.5524/100505

Trombidid mites have a unique lifecycle in which only the larval stage is ectoparasitic. In the superfamily Trombiculoidea ('chiggers'), the larvae feed preferentially on vertebrates, including humans. Species in the genus Leptotrombidium are vectors of a potentially fatal bacterial infection, scrub typhus, which affects 1 million people annually. Moreover, chiggers can cause pruritic dermatitis (trombiculiasis) in humans and domesticated animals. In the Trombidioidea (velvet mites), the larvae feed on other arthropods and are potential biological control agents for agricultural pests. Here, we present the first trombidid mites genomes, obtained both for a chigger, Leptotrombidium deliense, and for a velvet mite, Dinothrombium tinctorium.
Sequencing was performed using Illumina technology. A 180 Mb draft assembly for D. tinctorium was generated from two paired-end and one mate-pair library using a single adult specimen. ForL. deliense, a lower-coverage draft assembly (117 Mb) was obtained using pooled, engorged larvae with a single paired-end library. Remarkably, both genomes exhibited evidence of ancient lateral gene transfer from soil-derived bacteria or fungi. The transferred genes confer functions that are rare in animals, including terpene and carotenoid synthesis. Thirty-seven allergenic protein families were predicted in the L. deliense genome, of which nine were unique. Preliminary proteomic analyses identified several of these putative allergens in larvae.
Trombidid mite genomes appear to be more dynamic than those of other acariform mites. A priority for future research is to determine the biological function of terpene synthesis in this taxon and its potential for exploitation in disease control.

Additional details

Read the peer-reviewed publication(s):

Dong, X., Chaisiri, K., Xia, D., Armstrong, S. D., Fang, Y., Donnelly, M. J., … Makepeace, B. L. (2018). Genomes of trombidid mites reveal novel predicted allergens and laterally transferred genes associated with secondary metabolism. GigaScience, 7(12). doi:10.1093/gigascience/giy127

Accessions (data generated as part of this study):

PRIDE: PXD008346
BioProject: PRJNA377801
BioProject: PRJNA377800





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
Dt-prot1965070  Dinothrombium tinctorium Description:Dinothrombium tinctorium adult mites c...
Life stage:adult
Geographic location (country and/or sea,region):Th...
...
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Ld-prot299467scrub typhus mite  Leptotrombidium deliense Description:Leptotrombidium deliense larvae collec...
Life stage:engorged larvae
Specific host:Bandicota indica; Bandicota savilei;...
...
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SAMN064730821965070  Dinothrombium tinctorium Description:Adult Dinothrombium tinctorium mites c...
Life stage:adult
Geographic location (country and/or sea,region):Th...
...
+
SAMN06473100299467scrub typhus mite  Leptotrombidium deliense Description:Leptotrombidium deliense larvae collec...
Life stage:larvae
Specific host:Menetes berdmorei
...
+
Displaying 1-4 of 4 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
AlignmentsFASTA22.86 KB2018-09-27
AlignmentsFASTA44.81 KB2018-09-27
AlignmentsFASTA323.05 KB2018-09-27
AlignmentsFASTA1.43 MB2018-09-27
AlignmentsFASTA26.25 KB2018-09-27
AlignmentsFASTA19.16 KB2018-09-27
AlignmentsFASTA7.83 KB2018-09-27
AlignmentsFASTA16.98 KB2018-09-27
AlignmentsFASTA32.43 KB2018-09-27
AlignmentsFASTA21.05 KB2018-09-27
Displaying 1-10 of 45 File(s).
Funding body Awardee Award ID Comments
University Of Liverpool A C Darby
Bayer J W McGarry Animal Health division
Date Action
October 15, 2018 Dataset publish
November 3, 2018 Manuscript Link added : 10.1093/gigascience/giy127