Supporting data for "Toxins from scratch? - Diverse, multimodal gene origins in predatory robber flies indicate dynamic venom evolution in dipteran insects"

Dataset type: Genomic, Transcriptomic, Proteomic
Data released on June 19, 2019

Drukewitz SH; Bokelmann L; B Undheim EA; von Reumont BM (2019): Supporting data for "Toxins from scratch? - Diverse, multimodal gene origins in predatory robber flies indicate dynamic venom evolution in dipteran insects" GigaScience Database. http://dx.doi.org/10.5524/100612

DOI10.5524/100612

Venoms and the toxins they contain represent molecular adaptations that have evolved on numerous occasions throughout the animal kingdom. However, the processes that shape venom protein evolution are poorly understood because of the scarcity of whole genome data available for comparative analyses of venomous species. Here, we perform a broad comparative toxicogenomic analysis to gain insight into the genomic mechanisms of venom evolution in robber flies (Asilidae). We first sequenced a high-quality draft genome of the hymenopteran hunting robber fly Dasypogon diadema, analyzed its venom by a combined proteotranscriptomic approach, and compared our results to recently described robber fly venoms to assess the general composition and major components of asilid venom. We then applied a comparative genomics approach, based on one additional asilid genome, ten high-quality dipteran genomes, and two lepidopteran outgroup-genomes, to reveal the evolutionary mechanisms and origins of identified venom proteins in robber flies. While homologs were identified for 15 out of 30 predominant venom protein in the nonasilid genomes, the remaining 15 highly expressed venom proteins appear to be unique to robber flies. Our results reveal that the venom of D. diadema likely evolves in a multimodal fashion comprising 1) neofunctionalization after gene duplication, 2) expression-dependent co-option of proteins and 3) asilid lineage-specific orphan genes with enigmatic origin. The role of such orphan genes is currently being disputed in evolutionary genomics, but has not been discussed in the context of toxin evolution. Our results display an unexpected dynamic venom evolution in asilid insects, which contrasts the findings of the only other insect toxicogenomic evolutionary analysis, in parasitoid wasps (Hymenoptera), where toxin evolution is dominated by single gene co-option. These findings underpin the significance of further genomic studies to cover more neglected lineages of venomous taxa and to understand the importance of orphan genes as possible drivers for venom evolution.

Additional details

Read the peer-reviewed publication(s):

(PubMed: 31289835)

Accessions (data generated as part of this study):

PRIDE: PXD013358
BioProject: PRJNA361480
SRA: SRR7878513
SRA: SRR7878512





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
Dasy_Body_fem468822  Dasypogon diadema Description:DNA extracted from the muscle of a fem...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN06234164
...
+
Dasy_Body_male468822  Dasypogon diadema Description:RNA extracted from the muscle of a mal...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN06234163
...
+
Dasy_Proboscis_fem468822  Dasypogon diadema Description:RNA extracted from the proboscis of a ...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN09874252
...
+
Dasy_Proboscis_male468822  Dasypogon diadema Description:RNA extracted from the proboscis of a ...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN09874253
...
+
Dasy_VG_fem468822  Dasypogon diadema Description:RNA extracted from the venom gland of ...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN09874189
...
+
Dasy_VG_male468822  Dasypogon diadema Description:RNA extracted from the venom gland of ...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN09874251
...
+
EuRufi_Body1936067  Eutolmus rufibarbis Description:RNA extracted from the muscle of poole...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN06234161
...
+
EuRufi_VG1936067  Eutolmus rufibarbis Description:RNA extracted from the thoracic gland ...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN06234162
...
+
MaArt_Body1936065  Machimus arthriticus Description:RNA extracted from the muscle of poole...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN06234159
...
+
MaArt_VG1936065  Machimus arthriticus Description:RNA extracted from the thoracic gland ...
Alternative accession-BioProject:PRJNA361480
Alternative accession-BioSample:SAMN06234160
...
+
Displaying 1-10 of 10 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
Mass Spectrometry dataEXCEL310.37 KB2019-06-06
Mass Spectrometry dataUNKNOWN15.09 MB2019-06-06
Mass Spectrometry dataUNKNOWN4.34 MB2019-06-06
Mass Spectrometry dataUNKNOWN65.56 MB2019-06-06
Mass Spectrometry dataEXCEL538.31 KB2019-06-06
Mass Spectrometry dataUNKNOWN27.62 MB2019-06-06
Mass Spectrometry dataUNKNOWN4.27 MB2019-06-06
Mass Spectrometry dataUNKNOWN68.62 MB2019-06-06
OtherTEXT0.8 KB2019-06-06
AlignmentsFASTA145.62 KB2019-06-06
Displaying 1-10 of 203 File(s).
Funding body Awardee Award ID Comments
Deutsche Forschungsgemeinschaft B M von Reumont RE3454/4-1
Australian Research Council (AU) E A B Undheim DE160101142
Australian Research Council E A B Undheim DP160104025
Paul Scherer Institut B M von Reumont 20160644
Date Action
June 13, 2019 Dataset publish
June 24, 2019 Manuscript Link added : 10.1093/gigascience/giz081
October 14, 2022 Manuscript Link updated : 10.1093/gigascience/giz081