Supporting data for "A Chromosomal-Level Genome Assembly for the insect vector for Chagas disease, Triatoma rubrofasciata"
Dataset type: Genomic, Transcriptomic
Data released on July 01, 2019
Triatoma rubrofasciata is a widespread pathogen vector for Chagas disease, an illness that affects approximately seven million people worldwide. Despite of its importance to human health, its evolutionary origin has not been conclusively determined. A reference genome for T. rubrofasciata is not yet available.
We have sequenced the genome of a female T. rubrofasciata individual using a single molecular DNA sequencing technology (i.e., PacBio Sequel platform) and have successfully reconstructed a whole-genome (680 Mb) assembly that covers 90% of the nuclear genome (757 Mb). Through Hi-C analysis, we have reconstructed full-length chromosomes of this female individual that has 13 unique chromosomes (2n = 24 = 22 + X1 + X2) with a contig N50 of 2.72Mb and a scaffold N50 of 50.7 Mb. This genome has achieved a high base-level accuracy of 99.99%. This platinum-grade genome assembly has 12,691 annotated protein-coding genes. More than 95.1% BUSCO genes were single-copy completed, indicating a high level of completeness of the genome.
The platinum-grade genome assembly and its annotation provide valuable information for future in-depth comparative genomics studies including sexual determination analysis in T. rubrofasciata and the pathogenesis of Chagas disease.
Additional details
Read the peer-reviewed publication(s):
(PubMed: 31425588)
Genome browser:
https://trace.ncbi.nlm.nih.gov/Traces/study/?acc=PRJNA516044&go=go
Accessions (data generated as part of this study):
BioProject:
PRJNA516044
BioSample :
SRR8466737
BioSample :
SRR8466736
BioSample :
SRR8466756
BioSample :
SRR8468315
BioSample :
SRR8468316
Sample ID | Taxonomic ID | Common Name | Genbank Name | Scientific Name | Sample Attributes |
---|---|---|---|---|---|
NIPD-004 | 162384 | large kissing bug | Triatoma rubrofasciata | Description:DNA extracted from adult female Triato... Common name:large kissing bug Alternative accession-BioSample:SAMN10781786 ... + |