Supporting data for the chromosome-level genome assembly of the malaria vectors Anopheles coluzzii

Dataset type: Genomic
Data released on January 21, 2021

Zamyatin A; Avdeyev P; Liang J; Sharma A; Chen C; Lukyanchikova V; Alexeev N; Tu Z; Alekseyev MA; Sharakhov IV (2021): Supporting data for the chromosome-level genome assembly of the malaria vectors Anopheles coluzzii GigaScience Database. http://dx.doi.org/10.5524/100861

DOI10.5524/100861

Anopheles coluzzii belongs to the An. gambiae complex and is among the major malaria vectors in Sub-Saharan Africa. However, a chromosome-level reference genome assembly for this medically important species is not yet available.
In this study, we produced de novo chromosome-level genome assemblies for both An. coluzzii and An. arabiensis using the long-read Oxford Nanopore sequencing technology and the Hi-C scaffolding approach.
We obtained a 273.4 Mbp assembly for An. coluzzii consisting of three chromosome-scale scaffolds (X, 2, 3), complete mitochondrion, and unordered contigs identified as autosomal pericentromeric DNA, X pericentromeric DNA, and Y sequences. A comparison of our assembly with the existing assemblies for these species demonstrated that we obtained improved reference-quality genomes.
Together, the new assemblies of An. coluzzii and An. arabiensis allowed us to identify genomic coordinates for the breakpoint regions of fixed and polymorphic chromosomal inversions.
This new chromosome-level assembly will facilitate functional and population genomic studies and the presented assembly pipeline will accelerate progress toward creating high-quality genome references for other disease vectors.

Additional details

Read the peer-reviewed publication(s):

(PubMed: 33718948)

Accessions (data generated as part of this study):

BioProject: PRJNA634549

Accessions (data referenced by this study):

SRA: SRX3832577





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
An.coluzzi_Hi-C1518534  Anopheles coluzzii Analyte type:DNA
Alternative accession-BioSample:SAMN14451440
Alternative accession-BioProject:PRJNA615337
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An.coluzzii_adults1518534  Anopheles coluzzii Description:DNA extracted from 20 indivual male pu...
Analyte type:DNA
Alternative accession-BioSample:SAMN14999449
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Displaying 1-2 of 2 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
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Funding body Awardee Award ID Comments
National Institute of Health (NIH) IV Sharakhov R21AI135298
Government of the Russian Federation N Alexeev ITMO Fellowship and Professorship Program
National Institute of Food and Agriculture IV Sharakhov. 223822 Hatch project
Date Action
January 21, 2021 Dataset publish
February 22, 2021 Manuscript Link added : 10.1093/gigascience/giab017
November 29, 2021 Manuscript Link updated : 10.1093/gigascience/giab017