Supporting data for "X-ray microtomography-based atlas of mouse cranial development"

Dataset type: Imaging
Data released on February 01, 2021

Matula J; Tesarova M; Zikmund T; Kaucka M; Adameyko I; Kaiser J (2021): Supporting data for "X-ray microtomography-based atlas of mouse cranial development" GigaScience Database. http://dx.doi.org/10.5524/100862

DOI10.5524/100862

X-ray microtomography (μCT) has become an invaluable tool for non-destructive analysis of biological samples in the field of developmental biology. Mouse embryos are a typical model for investigation of human developmental diseases. By obtaining 3-D high resolution scans of the mouse embryo heads, we gain valuable morphological information about the structures prominent in the development of future face, brain and sensory organs. The development of facial skeleton tracked in these μCT data provides a valuable background for further studies of congenital craniofacial pathologies and normal development. In this work, re-usable tomographic data from 7 full 3-D scans of mouse embryo heads are presented and made publicly available. The ages of these embryos range from E12.5 to E18.5. The samples were stained by phosphotungstic acid prior to scanning, which greatly enhanced the contrast of various tissues in the reconstructed images and enabled precise segmentation. The images were obtained on a lab-based μCT system. Furthermore, we provide manually segmented masks of mesenchymal condensations (for E12.5 and E13.5) and cartilage present in the nasal capsule of the scanned embryos. We present a comprehensive dataset of X-ray 3-D computed tomography images of the developing mouse head with high-quality manual segmentation masks of cartilaginous nasal capsules. The provided μCT images can be used for studying any other major structure within the developing mouse heads. The high quality of the manually segmented models of nasal capsules may be instrumental to understand the complex process of the development of the face in mouse model.





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
E125_mus_musculus10090Mousehouse mouseMus musculus Description:Embryonic mouse head specimen at E12.5...
Life stage:Embryo [Theiler stage:20]
Age:E12.5
...
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E135_mus_musculus10090Mousehouse mouseMus musculus Description:Embryonic mouse head specimen at E13.5...
Life stage:Embryo [Theiler stage:21]
Age:E13.5
...
+
E145_mus_musculus10090Mousehouse mouseMus musculus Description:Embryonic mouse head specimen at E14.5...
Life stage:Embryo [Theiler stage:23]
Age:E14.5
...
+
E155_mus_musculus10090Mousehouse mouseMus musculus Description:Embryonic mouse head specimen at E15.5...
Life stage:Embryo [Theiler stage:24]
Age:E15.5
...
+
E165_mus_musculus10090Mousehouse mouseMus musculus Description:Embryonic mouse head specimen at E16.5...
Life stage:Embryo [Theiler stage:25]
Age:E16.5
...
+
E175_mus_musculus10090Mousehouse mouseMus musculus Description:Embryonic mouse head specimen at E17.5...
Life stage:Embryo [Theiler stage:26]
Age:E17.5
...
+
E185_mus_musculus10090Mousehouse mouseMus musculus Description:Embryonic mouse head specimen at E18.5...
Life stage:Embryo [Theiler stage:26]
Age:E18.5
...
+
Displaying 1-7 of 7 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
E125_mus_musculusimageGZIP1.01 GB2021-01-28
E125_mus_musculustextTEXT0.04 KB2021-01-28
E125_mus_musculusimageGZIP5.83 MB2021-01-28
E125_mus_musculusimageSTL47.55 MB2021-01-28
E135_mus_musculusimageGZIP1.36 GB2021-01-28
E135_mus_musculustextTEXT0.04 KB2021-01-28
E135_mus_musculusimageGZIP6.44 MB2021-01-28
E135_mus_musculusimageSTL47.53 MB2021-01-28
E145_mus_musculusimageGZIP518.94 MB2021-01-28
E145_mus_musculustextTEXT0.04 KB2021-01-28
Displaying 1-10 of 36 File(s).
Funding body Awardee Award ID Comments
Ministry of Education Youth and Sports J Matula LQ1601 CEITEC 2020
Ministry of Education Youth and Sports J Matula LM2018110 CzechNanoLab Research Infrastructure
Brno University of Technology J Matula VUT-J-20-6477 Vnitřní projekty VUT
Brno University of Technology M Tesarova VUT-J-20-6317 Vnitřní projekty VUT
The Brno City Municipality M Tesarova Brno Ph.D. Talent Scholarship

3D Models:

Date Action
February 1, 2021 Dataset publish
February 8, 2021 Manuscript Link added : 10.1093/gigascience/giab012