Supporting data for "De novo screening of disease-resistant genes from the chromosome-level genome of rare minnow using CRISPR-cas9 random mutation."
Dataset type: Genomic
Data released on October 22, 2021
Mutants are important for the discovery of functional genes and creation of germplasm resources. Mutant acquisition depends on the efficiency of mutation technology and screening methods. CRISPR-Cas9 technology is an efficient gene editing technology mainly used for editing a few genes or target sites, which has not been applied for the construction of random mutant libraries and for the de novo discovery of functional genes.
In this study, we first sequenced and assembled the chromosome-level genome of wild-type rare minnow as a susceptible model of hemorrhagic disease, obtained a 956.05 M genome sequence, assembled the sequence into 25 chromosomes, and annotated 26,861 protein-coding genes. Thereafter, CRISPR-Cas9 technology was applied to randomly mutate the whole genome of rare minnow with the conserved bases (TATAWAW and ATG) of the promoter and coding regions as the target sites. The survival rate of hemorrhagic disease in the rare minnow gradually increased from 0% (the entire wild-type population died after infection) to 38.24% (F3 generation). Finally, seven susceptible genes were identified via genome comparative analysis and cell-level verification based on the rare minnow genome.
The results provided the genomic resources for wild-type rare minnow, and confirmed that the random mutation system designed using CRISPR-Cas9 technology in this study is simple and efficient, and is suitable for the de novo discovery of functional genes and creation of a germplasm related to quality traits.
Additional details
Read the peer-reviewed publication(s):
(PubMed: 34849868)
Accessions (data generated as part of this study):
BioProject:
PRJNA732062
BioProject:
PRJNA732511
BioProject:
PRJNA613868
BioProject:
PRJNA715041
Sample ID | Taxonomic ID | Common Name | Genbank Name | Scientific Name | Sample Attributes |
---|---|---|---|---|---|
C | 143606 | Gobiocypris rarus | Description:DNA extracted from a mixed male & fema... Alternative accession-BioSample:SAMN14421215 Geographic location (country and/or sea,region):Ch... ... + | ||
DN1 | 143606 | Gobiocypris rarus | Description:DNA extracted from the muscle of an ad... Alternative accession-BioSample:SAMN19307644 Geographic location (country and/or sea,region):Ch... ... + | ||
GR2020 | 143606 | Gobiocypris rarus | Description:DNA extracted from the muscle of an ad... Alternative accession-BioSample:SAMN18323793 Geographic location (country and/or sea,region):Ch... ... + | ||
L7 | 143606 | Gobiocypris rarus | Description:DNA extracted from the caudal fin of a... Alternative accession-BioSample:SAMN19321769 Geographic location (country and/or sea,region):Ch... ... + | ||
P1 | 143606 | Gobiocypris rarus | Description:DNA extracted from the caudal fin of a... Alternative accession-BioSample:SAMN19321770 Geographic location (country and/or sea,region):Ch... ... + | ||
P2 | 143606 | Gobiocypris rarus | Description:DNA extracted from the caudal fin of a... Alternative accession-BioSample:SAMN19321771 Analyte type:DNA ... + | ||
P3 | 143606 | Gobiocypris rarus | Description:DNA extracted from the caudal fin of a... Alternative accession-BioSample:SAMN19321772 Analyte type:DNA ... + | ||
P4 | 143606 | Gobiocypris rarus | Description:DNA extracted from the caudal fin of a... Alternative accession-BioSample:SAMN19321773 Analyte type:DNA ... + | ||
P5 | 143606 | Gobiocypris rarus | Description:DNA extracted from the caudal fin of a... Alternative accession-BioSample:SAMN19321774 Analyte type:DNA ... + | ||
S1 | 143606 | Gobiocypris rarus | Description:DNA extracted from the caudal fin of a... Alternative accession-BioSample:SAMN19321768 Geographic location (country and/or sea,region):Ch... ... + |