Supporting data for "A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana"

Dataset type: Genomic, Transcriptomic
Data released on May 04, 2017

Nowell RW; Elsworth B; Oostra V; Zwaan BJ; Wheat CW; Saastamoinen M; Saccheri IJ; van’t-Hof AE; Wasik BR; Connahs H; Aslam ML; Kumar S; Challis RJ; Monteiro A; Brakefield PM; Blaxter M (2017): Supporting data for "A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana" GigaScience Database. http://dx.doi.org/10.5524/100280

DOI10.5524/100280

The mycalesine butterfly Bicyclus anynana, the ‘Squinting bush brown’, is a model organism in the study of lepidopteran ecology, development and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Eight libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology. 128 Gb raw Illumina data were filtered to 124 Gb and assembled to a final size of 475 Mb (~260X assembly coverage). Contigs were scaffolded using mate-pair, transcriptome and PacBio data into 10,800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements, and encodes a total of 22,642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence for Bicyclus anynana. The genome assembly and annotated gene models are available at LepBase (http://ensembl.lepbase.org/index.html).

Additional details

Read the peer-reviewed publication(s):

Nowell, R. W., Elsworth, B., Oostra, V., Zwaan, B. J., Wheat, C. W., Saastamoinen, M., … Blaxter, M. (2017). A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana. GigaScience, 6(7), 1–7. doi:10.1093/gigascience/gix035

Additional information:

http://ensembl.lepbase.org/index.html

Accessions (data included in GigaDB):

BioProject: PRJEB8426

Accessions (data not in GigaDB):

BioProject: PRJNA376691





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
ERS1505521110368 squinting bush brownBicyclus anynana Description:DNA extracted from female pupae for Pa...
Environment (biome):terrestrial biome [ENVO:000004...
Environment (feature):savannah [ENVO:ENVO:00000261...
...
+
ERS1469840110368 squinting bush brownBicyclus anynana Description:DNA extracted from female pupae for Il...
Environment (biome):terrestrial biome [ENVO:000004...
Environment (feature):savannah [ENVO:ENVO:00000261...
...
+
ERS1469841110368 squinting bush brownBicyclus anynana Description:DNA extracted from female pupae for Il...
Environment (biome):terrestrial biome [ENVO:000004...
Environment (feature):savannah [ENVO:ENVO:00000261...
...
+
ERS947087110368 squinting bush brownBicyclus anynana Description:DNA extracted from female pupae for Il...
Environment (biome):terrestrial biome [ENVO:000004...
Environment (feature):savannah [ENVO:ENVO:00000261...
...
+
Displaying 1-4 of 4 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
Coding sequenceFASTA7.04 MB2017-02-28
annotationGFF4.95 MB2017-02-28
protein sequenceFASTA4.7 MB2017-02-28
Genome sequenceFASTA127.93 MB2017-02-28
otherTAR27.75 MB2017-02-28
otherTAR1.03 MB2017-02-28
ReadmeTEXT2.07 KB2017-02-28
Repeat sequenceTAR397.38 MB2017-02-28
Displaying 1-8 of 8 File(s).
Date Action
May 4, 2017 Dataset publish
June 27, 2017 Manuscript Link added : 10.1093/gigascience/gix035