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Data released on April 17, 2017

Data supporting analysis of evolution of SET genes in insects

Jiang, F; Kang, L; Liu, Q; Song, T; Wang, H; Wang, X; Wang, Y; Yang, M; Zhang, J (2017): Data supporting analysis of evolution of SET genes in insects GigaScience Database. http://dx.doi.org/10.5524/100299 RIS BibTeX Text

The SET domain is an evolutionarily conserved motif present in histone lysine methyltransferases, which are important in the regulation of chromatin and gene expression in animals. In this study, we searched for SET domain-containing genes (SET genes) in all of the 147 arthropod genomes sequenced so far to understand the evolutionary history by which SET domain have evolved in insects. Phylogenetic and ancestral state reconstruction analysis revealed an arthropod-specific SET gene family, named SmydA, which is ancestral to arthropod animals and specifically diversified during insect evolution. Considering that pseudogenization is the most probable fate of the new emerging gene copies, we provided experimental and evolutionary evidence to demonstrate their essential functions. Fluorescence in situ hybridization analysis and in vitro methyltransferase activity assays showed that the SmydA-2 gene was transcriptionally active and retained the original histone methylation activity. Expression knockdown by RNA interference significantly increased mortality, implying that the SmydA genes may be essential for insect survival. We further showed predominantly strong purifying selection on the SmydA gene family and a potential association between the regulation of gene expression and insect phenotypic plasticity by transcriptome analysis. Overall, these data suggest that the SmydA gene family retains essential functions that may possibly define novel regulatory pathways in insects. This work provides insights into the roles of lineage-specific domain duplication in insect evolution.

Contact Submitter

Related manuscripts:

doi:10.1093/gigascience/gix031

Additional information:

http://159.226.67.242:8080/

Keywords:

insects domain gene duplication histone modification 

Genomic

http://gigadb.org/images/data/cropped/100299.jpg

Funding:

  • Funding body - National Natural Science Foundation of China
  • Award ID - 31430023
  • Awardee - Le Kang
  • Funding body - National Natural Science Foundation of China
  • Award ID - 31672353
  • Awardee - Feng Jiang
  • Funding body - National Natural Science Foundation of China
  • Award ID - 31301915
  • Awardee - Feng Jiang
  • Funding body - National Natural Science Foundation of China
  • Award ID - 31472047
  • Awardee - Xianhui Wang
  • Funding body - National Natural Science Foundation of China
  • Award ID - 31472051
  • Awardee - Meiling Yang

Samples: Table Settings

Columns:

Common Name
Scienfic Name
Sample Attributes
Taxonomic ID
Genbank Name

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
Acromyrmex echinatior (large worker)103372Leafcutter antPanamanian leafcutter antAcromyrmex echinatior Description:RNA-seq
Tissue:large worker heads
Alternative accession-GEOsample:GSM1248674
...
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Acromyrmex echinatior (Queen)103372Leafcutter antPanamanian leafcutter antAcromyrmex echinatior Description:RNA-seq
Tissue:gyne heads
Alternative accession-GEOsample:GSM1248668
...
+
asexual morphs A. pisum7029 pea aphidAcyrthosiphon pisum Description:RNA-seq
Tissue:Pea aphid whole body
Age:2 day old adult
...
+
gregarious locusts7004 migratory locustLocusta migratoria Description:RNA-seq
Tissue:Whole body
Alternative accession-BioSample:SAMN00016436
...
+
queen honey bee7460European honey beehoney beeApis mellifera Description:RNA-seq
Tissue:Whole body
Developmental stage:L5 Larvae
...
+
sexual morphs A. pisum7029 pea aphidAcyrthosiphon pisum Description:RNA-seq
Tissue:Pea aphid whole body
Age:2-6 day old adult
...
+
solitary locusts7004 migratory locustLocusta migratoria Description:RNA-seq
Tissue:Whole body
Alternative accession-BioSample:SAMN00016435
...
+
worker honey bee7460European honey beehoney beeApis mellifera Description:RNA-seq
Tissue:Whole body
Developmental stage:L5 Larvae
...
+
Displaying 1-8 of 8 Sample(s).

Files: (FTP site) Table Settings

Columns:

File Description
Sample ID
File Type
File Format
Size
Release Date
Download Link
File Attributes

File NameSample IDFile TypeFile FormatSizeRelease Date 
AlignmentsUNKNOWN289.17 KB2017-04-10
AlignmentsUNKNOWN3.11 KB2017-04-10
AlignmentsUNKNOWN429.56 KB2017-04-10
AlignmentsUNKNOWN340.03 KB2017-04-10
AlignmentsUNKNOWN148.42 KB2017-04-10
AlignmentsUNKNOWN6.01 KB2017-04-10
AlignmentsUNKNOWN290.68 KB2017-04-10
AlignmentsUNKNOWN64.54 MB2017-04-10
AlignmentsUNKNOWN2.96 KB2017-04-10
AlignmentsUNKNOWN3.75 KB2017-04-10
Displaying 1-10 of 49 File(s).

History:

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