Supporting data for "Transcriptome Analysis of the Response of Burmese Python to Digestion"

Dataset type: Transcriptomic, Proteomic
Data released on July 06, 2017

Duan J; Enghild JJ; Lauridsen SE; Sanggaard KW; Schauser L; Schierup MH; Wang T (2017): Supporting data for "Transcriptome Analysis of the Response of Burmese Python to Digestion" GigaScience Database.


Exceptional and extreme feeding behaviour makes the Burmese python (Python bivittatus) an interesting model to study physiological remodelling and metabolic adaptation in response to refeeding after prolonged starvation. In this study, we used transcriptome sequencing of five visceral organs during fasting as well as 24h and 48h after ingestion of a large meal to unravel the postprandial changes in Burmese pythons. We first used the pooled data to perform a de novo assembly of the transcriptome and supplemented this with a proteomic survey of enzymes in the plasma and gastric fluid.
We constructed a high-quality transcriptome with 34,423 transcripts of which 19,713 (57%) were annotated. Among highly expressed genes (FPKM>100 in one tissue) we found the transition from fasting to digestion was associated with differential expression of 43 genes in the heart, 206 genes in the liver, 114 genes in the stomach, 89 genes in the pancreas and 158 genes in the intestine. We interrogated the function of these genes to test previous hypotheses on the response to feeding. We also used the transcriptome to identify 314 secreted proteins in the gastric fluid of the python.
Digestion was associated with an upregulation of genes related to metabolic processes, and translational changes therefore appears to support the postprandial rise in metabolism. We identify stomach-related proteins from a digesting individual and demonstrate that the sensitivity of modern LC-MS/MS equipment allows the identification of gastric juice proteins that are present during digestion.

Additional details

Read the peer-reviewed publication(s):

Duan, J., Sanggaard, K. W., Schauser, L., Lauridsen, S. E., Enghild, J. J., Schierup, M. H., & Wang, T. (2017). Transcriptome analysis of the response of Burmese python to digestion. GigaScience, 6(8), 1–18. doi:10.1093/gigascience/gix057

Accessions (data included in GigaDB):

PRIDE: PXD006665
BioProject: PRJNA343735

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
SRS1706099176946 Burmese pythonPython bivittatus Description:Heart tissue cDNA from Burmese Python ...
Alternative accession-BioSample:SAMN05792571
SRS1706100176946 Burmese pythonPython bivittatus Description:Liver tissue cDNA from Burmese Python ...
Alternative accession-BioSample:SAMN05792574
SRS1706101176946 Burmese pythonPython bivittatus Description:Stomach tissue cDNA from Burmese Pytho...
Alternative accession-BioSample:SAMN05792580
SRS1706102176946 Burmese pythonPython bivittatus Description:Intestine tissue cDNA from Burmese Pyt...
Alternative accession-BioSample:SAMN05792577
SRS1706103176946 Burmese pythonPython bivittatus Description:Pancreas tissue cDNA from Burmese Pyth...
Alternative accession-BioSample:SAMN05792583
Displaying 21-25 of 25 Sample(s).

File NameSample IDData TypeFile FormatSizeRelease Date 
ReadmeTEXT7.65 KB2017-06-27
Expression dataUNKNOWN3.42 MB2017-06-27
Expression dataUNKNOWN3.42 MB2017-06-27
Expression dataUNKNOWN3.33 MB2017-06-27
Expression dataUNKNOWN3.33 MB2017-06-27
Expression dataUNKNOWN3.41 MB2017-06-27
Expression dataUNKNOWN3.38 MB2017-06-27
Expression dataUNKNOWN3.38 MB2017-06-27
Expression dataUNKNOWN3.43 MB2017-06-27
BLASTTEXT123.82 MB2017-06-27
Displaying 1-10 of 71 File(s).
Funding body Awardee Award ID Comments
Danish Research Council for Strategic Research T Wang 09-067076
Danish Council for Independent Research JJ Enghild DFF-12-126136
Date Action
July 6, 2017 Dataset publish
July 14, 2017 File readme.txt updated
October 2, 2017 Manuscript Link added : 10.1093/gigascience/gix057