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Data released on July 06, 2017

Supporting data for "Transcriptome Analysis of the Response of Burmese Python to Digestion"

Duan, J; Enghild, J, J; Lauridsen, S, E; Sanggaard, K, W; Schauser, L; Schierup, M, H; Wang, T (2017): Supporting data for "Transcriptome Analysis of the Response of Burmese Python to Digestion" GigaScience Database. http://dx.doi.org/10.5524/100320 RIS BibTeX Text

Exceptional and extreme feeding behaviour makes the Burmese python (Python bivittatus) an interesting model to study physiological remodelling and metabolic adaptation in response to refeeding after prolonged starvation. In this study, we used transcriptome sequencing of five visceral organs during fasting as well as 24h and 48h after ingestion of a large meal to unravel the postprandial changes in Burmese pythons. We first used the pooled data to perform a de novo assembly of the transcriptome and supplemented this with a proteomic survey of enzymes in the plasma and gastric fluid.
We constructed a high-quality transcriptome with 34,423 transcripts of which 19,713 (57%) were annotated. Among highly expressed genes (FPKM>100 in one tissue) we found the transition from fasting to digestion was associated with differential expression of 43 genes in the heart, 206 genes in the liver, 114 genes in the stomach, 89 genes in the pancreas and 158 genes in the intestine. We interrogated the function of these genes to test previous hypotheses on the response to feeding. We also used the transcriptome to identify 314 secreted proteins in the gastric fluid of the python.
Digestion was associated with an upregulation of genes related to metabolic processes, and translational changes therefore appears to support the postprandial rise in metabolism. We identify stomach-related proteins from a digesting individual and demonstrate that the sensitivity of modern LC-MS/MS equipment allows the identification of gastric juice proteins that are present during digestion.

Contact Submitter

Related manuscripts:

doi:10.1093/gigascience/gix057

Accessions (data included in GigaDB):

PRIDE: PXD006665
BioProject: PRJNA343735

Keywords:

Burmese Python transcriptome tissue expression digestion pathway proteome 

Transcriptomic, Proteomic

http://gigadb.org/images/data/cropped/100320.jpg

Funding:

  • Funding body - Danish Research Council for Strategic Research
  • Award ID - 09-067076
  • Awardee - T Wang
  • Funding body - Danish Council for Independent Research
  • Award ID - DFF-12-126136
  • Awardee - JJ Enghild

Samples: Table Settings

Columns:

Common Name
Scienfic Name
Sample Attributes
Taxonomic ID
Genbank Name

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
SRS1706080176946 Burmese pythonPython bivittatus Description:Heart tissue cDNA from Burmese Python ...
Alternative accession-BioSample:SAMN05792570
Tissue:Heart
...
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SRS1706081176946 Burmese pythonPython bivittatus Description:Heart tissue cDNA from Burmese Python ...
Alternative accession-BioSample:SAMN05792572
Tissue:Heart
...
+
SRS1706082176946 Burmese pythonPython bivittatus Description:Liver tissue cDNA from Burmese Python ...
Alternative accession-BioSample:SAMN05792575
Tissue:Liver
...
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SRS1706083176946 Burmese pythonPython bivittatus Description:Heart tissue cDNA from Burmese Python ...
Alternative accession-BioSample:SAMN05792856
Tissue:Heart
...
+
SRS1706084176946 Burmese pythonPython bivittatus Description:Pancreas tissue cDNA from Burmese Pyth...
Alternative accession-BioSample:SAMN05792584
Tissue:Pancreas
...
+
SRS1706085176946 Burmese pythonPython bivittatus Description:Stomach tissue cDNA from Burmese Pytho...
Alternative accession-BioSample:SAMN05792581
Tissue:Stomach
...
+
SRS1706086176946 Burmese pythonPython bivittatus Description:Intestine tissue cDNA from Burmese Pyt...
Alternative accession-BioSample:SAMN05792578
Tissue:Intestine
...
+
SRS1706087176946 Burmese pythonPython bivittatus Description:Liver tissue cDNA from Burmese Python ...
Alternative accession-BioSample:SAMN05792857
Tissue:Liver
...
+
SRS1706088176946 Burmese pythonPython bivittatus Description:Stomach tissue cDNA from Burmese Pytho...
Alternative accession-BioSample:SAMN05792858
Tissue:Stomach
...
+
SRS1706079176946 Burmese pythonPython bivittatus Description:Liver tissue cDNA from Burmese Python ...
Alternative accession-BioSample:SAMN05792573
Tissue:Liver
...
+
Displaying 1-10 of 25 Sample(s).

Files: (FTP site) Table Settings

Columns:

File Description
Sample ID
File Type
File Format
Size
Release Date
Download Link
File Attributes

File NameSample IDFile TypeFile FormatSizeRelease Date 
Expression dataUNKNOWN3.4 MB2017-06-27
BLASTTEXT123.82 MB2017-06-27
ReadmeTEXT7.65 KB2017-06-27
Expression dataUNKNOWN3.42 MB2017-06-27
Expression dataUNKNOWN3.42 MB2017-06-27
Expression dataUNKNOWN3.33 MB2017-06-27
Expression dataUNKNOWN3.33 MB2017-06-27
Expression dataUNKNOWN3.41 MB2017-06-27
Expression dataUNKNOWN3.38 MB2017-06-27
transcriptome sequenceFASTA37.29 MB2017-06-27
Displaying 1-10 of 71 File(s).

History:

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