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Data released on January 05, 2018

Supporting data for "Giardia Secretome Highlights Secreted Tenascins as a Key Component of Pathogenesis"

Dubourg, A; Xia, D; Winpenny, J, P; Al-Naimi, S; Bouzid, M; Sexton, D, W; Wastling, J, M; Hunter, P, R; Tyler, K, M (2018): Supporting data for "Giardia Secretome Highlights Secreted Tenascins as a Key Component of Pathogenesis" GigaScience Database. http://dx.doi.org/10.5524/100381 RIS BibTeX Text

Giardia is a protozoan parasite of public health relevance that causes gastroenteritis in a wide range of hosts. Two genetically distinct lineages (assemblages A and B) are responsible for the human disease. Although it is clear that differences in virulence occur, pathogenesis and virulence of Giardia remains poorly understood. The genome of Giardia is believed to contain ORFs which could encode as many as 6,000 proteins. By successfully applying quantitative proteomic analyses to the whole parasite and to the supernatants derived from parasite culture of assemblages A and B, we confirm expression of ~1,600 proteins from each assemblage, the vast majority of which being common to both lineages. To look for signature enrichment of secreted proteins, we considered the ratio of proteins in the supernatant compared with the pellet which defined a small group of enriched proteins, putatively secreted at a steady state by cultured growing trophozoites of both assemblages. This secretome is enriched with proteins annotated to have N-terminal signal peptide. The most abundant secreted proteins include known virulence factors such as cathepsin B cysteine proteases and members of a Giardia superfamily of cysteine rich proteins which comprises VSPs, HCMPs and a new class of virulence factors, the Giardia tenascins. We demonstrate that physiological function of human enteric epithelial cells is disrupted by such soluble factors even in the absence of the trophozoites. We are able to propose a straightforward model of Giardia pathogenesis incorporating key roles for the major Giardia derived soluble mediators.

Contact Submitter

Read the peer-reviewed publication(s):

Dubourg, A., Xia, D., Winpenny, J. P., Al Naimi, S., Bouzid, M., Sexton, D. W., … Tyler, K. M. (2018). Giardia secretome highlights secreted tenascins as a key component of pathogenesis. GigaScience, 7(3). doi:10.1093/gigascience/giy003

Additional information:

http://giardiadb.org/giardiadb/app/record/dataset/DS_fce62acf01

http://giardiadb.org/giardiadb/app/record/dataset/DS_a800bfc55b

Protocols.io:

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Accessions (data included in GigaDB):

PRIDE: PXD004398

Keywords:

quantitative proteomics tenascin cysteine protease enteric pathogen giardia secretion proteomics 

Proteomic, Electrophysiology

http://gigadb.org/images/data/cropped/100381.jpg

Funding:

  • Funding body - European Commission
  • Award ID - FP7/2007-2013
  • Comment - European Union Seventh Framework Programme
  • Funding body - European Commission
  • Award ID - FP7/2007-2011
  • Comment - European Union Seventh Framework Programme

Samples: Table Settings

Columns:

Common Name
Scienfic Name
Sample Attributes
Taxonomic ID
Genbank Name

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
ATCC_505811394984  Giardia intestinalis assemblage B Description:Electrophysiology assay on Giardia ass...
Species-a:Giardia assemblage B
Life stage:trophozoite
...
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ATCC_505811394984  Giardia intestinalis assemblage B Description:Proteomic analysis of Giardia assembla...
Species-a:Giardia assemblage B
Life stage:trophozoite
...
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ATCC_505811394984  Giardia intestinalis assemblage B Description:Proteomic analysis of Giardia assembla...
Species-a:Giardia assemblage B
Life stage:trophozoite
...
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ATCC_50803941442  Giardia intestinalis assemblage A Description:Proteomic analysis of Giardia assembla...
Species-a:Giardia assemblage A
Life stage:trophozoite
...
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ATCC_50803941442  Giardia intestinalis assemblage A Description:Proteomic analysis of Giardia assembla...
Species-a:Giardia assemblage A
Life stage:trophozoite
...
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ATCC_50803941442  Giardia intestinalis assemblage A Description:Electrophysiology assay on Giardia ass...
Species-a:Giardia assemblage A
Life stage:trophozoite
...
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Electro_assay_control5741  Giardia intestinalis Description:Electrophysiology assay on Giardia int...
Species-a:unknown
Life stage:trophozoite
...
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Electro_assay_patient5741  Giardia intestinalis Description:Electrophysiology assay on Giardia int...
Species-a:unknown
Life stage:trophozoite
...
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Displaying 1-8 of 8 Sample(s).

Files: (FTP site) Table Settings

Columns:

File Description
Sample ID
Data Type
File Format
Size
Release Date
Download Link
File Attributes

File NameSample IDData TypeFile FormatSizeRelease Date 
AlignmentsFASTA-aln1.61 MB2017-12-18
Displaying 11-11 of 11 File(s).

History:

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