Supporting data for "Comparative whole genome DNA methylation profiling of cattle sperm and somatic tissues reveals striking hypomethylated patterns in sperm"

Dataset type: Epigenomic
Data released on March 30, 2018

Zhou Y; Connor EE; Bickhart DM; Li C; Baldwin RL; Schroeder SG; Rosen BD; Yang L; Van Tassell CP; Liu GE (2018): Supporting data for "Comparative whole genome DNA methylation profiling of cattle sperm and somatic tissues reveals striking hypomethylated patterns in sperm" GigaScience Database. http://dx.doi.org/10.5524/100424

DOI10.5524/100424

Compared to somatic cells, sperm cells undergo nearly complete reprogramming of DNA methylation and exchange histones by protamine. Although sperm DNA methylation has been studied in humans and other model species, as the most beneficiary species of artificial insemination, its status in cattle is largely unknown. Using whole-genome bisulfite sequencing (WGBS), we profiled the DNA methylome of cattle sperm through comparison with somatic cells from three bovine tissues (mammary gland, brain, and blood). Large differences between cattle sperm and somatic cells were observed in the methylation patterns of global CpGs, pericentromeric satellites, partially methylated domains (PMDs), hypomethylated regions (HMRs), and common repeats. As expected and like other species, we observed low methylation in the promoter regions and high methylation in the bodies of active genes. We detected selective hypomethylation of megabase domains of centromeric satellite clusters, which may be related to chromosome segregation during meiosis and their rapid transcriptional activation upon fertilization. We found more PMDs in sperm cells than in the somatic cells and identified meiosis-related genes like KIF2B and REPIN1, which are hypomethylated in sperm but hypermethylated in somatic cells. Besides the common HMRs around gene promoters which showed substantial differences between sperm and somatic cells, the sperm-specific HMRs also targeted to distinct spermatogenesis-related genes, including BOLL, MAEL, ASZ1, SYCP3, CTCFL, MND1, SPATA22, PLD6, DDX4, RBBP8, FKBP6, and SYCE1. Although common repeats were heavily methylated in both sperm and somatic cells, some hypomethylated repeats were enriched in gene promoters with large variations among tissues. For example, some young Bov-A2 repeats, which belong to the SINE family, were hypomethylated and could affect the promoter structures by introducing new regulatory elements. Our study provides a comprehensive resource for bovine sperm epigenomic research and enables new discoveries about DNA methylation and its role in male fertility.

Additional details

Read the peer-reviewed publication(s):

Zhou, Y., Connor, E. E., Bickhart, D. M., Li, C., Baldwin, R. L., Schroeder, S. G., … Liu, G. E. (2018). Comparative whole genome DNA methylation profiling of cattle sperm and somatic tissues reveals striking hypomethylated patterns in sperm. GigaScience, 7(5). doi:10.1093/gigascience/giy039

Accessions (data included in GigaDB):

BioProject: PRJNA417285





Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
SAMN079821289913bovinecattleBos taurus Description:Genomic DNA isolated from semen straws...
Sex:male
Tissue:sperm
...
+
SAMN079821299913bovinecattleBos taurus Description:Genomic DNA isolated from the mammary ...
Sex:female
Tissue:mammary gland
...
+
SAMN079821309913bovinecattleBos taurus Description:Genomic DNA isolated from the mammary ...
Sex:female
Tissue:mammary gland
...
+
SAMN079821319913bovinecattleBos taurus Description:Genomic DNA isolated from the prefront...
Sex:female
Tissue:prefrontal cortex
...
+
SAMN079821329913bovinecattleBos taurus Description:Genomic DNA isolated from semen straws...
Sex:male
Tissue:sperm
...
+
SAMN079821339913bovinecattleBos taurus Description:Genomic DNA isolated from semen straws...
Sex:male
Tissue:sperm
...
+
SAMN079821349913bovinecattleBos taurus Description:Genomic DNA isolated from semen straws...
Sex:male
Tissue:sperm
...
+
SAMN079821359913bovinecattleBos taurus Description:Genomic DNA isolated from the prefront...
Sex:female
Tissue:prefrontal cortex
...
+
SAMN079821369913bovinecattleBos taurus Description:Genomic DNA isolated from whole blood ...
Sex:female
Tissue:blood
...
+
SAMN079821379913bovinecattleBos taurus Description:Genomic DNA isolated from whole blood ...
Sex:female
Tissue:blood
...
+
Displaying 1-10 of 10 Sample(s).




File NameSample IDData TypeFile FormatSizeRelease Date 
ReadmeTEXT3.66 KB2018-03-14
Tabular DataUNKNOWN2.05 MB2018-03-14
Tabular DataUNKNOWN0.55 KB2018-03-14
Tabular DataUNKNOWN1.48 KB2018-03-14
Tabular DataUNKNOWN0.39 KB2018-03-14
Tabular DataUNKNOWN155.03 KB2018-03-14
Tabular DataUNKNOWN0.79 KB2018-03-14
Tabular DataUNKNOWN1.76 KB2018-03-14
Tabular DataUNKNOWN11.48 KB2018-03-14
Tabular DataUNKNOWN4.6 KB2018-03-14
Displaying 1-10 of 11 File(s).
Funding body Awardee Award ID Comments
National Institute of Food and Agriculture George Liu 2013-67015-20951
US-Israel Binational Agricultural Research and Development (BARD) Fund George Liu US-4997-17
Date Action
March 30, 2018 Dataset publish
July 4, 2018 Manuscript Link added : 10.1093/gigascience/giy039