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Data released on May 15, 2018

Supporting data for "The Long Noncoding RNA Landscape of Neuroendocrine Prostate Cancer and its Clinical Implications"

Ramnarine, V, R; Alshalalfa, M; Mo, F; Nabavi, N; Erho, N; Takhar, M; Shukin, R; Brahmbhatt, S; Gawronski, A; Kobelev, M; Nouri, M; Lin, D; Tsai, H; Lotan, T, L; Karnes, R, J; Rubin, M, A; Zoubeidi, A; Gleave, M, E; Sahinalp, C; Wyatt, A, W; Volik, S, V; Beltran, H; Davicioni, E; Wang, Y; Collins, C, C (2018): Supporting data for "The Long Noncoding RNA Landscape of Neuroendocrine Prostate Cancer and its Clinical Implications" GigaScience Database. http://dx.doi.org/10.5524/100443 RIS BibTeX Text

Treatment induced neuroendocrine prostate cancer (tNEPC) is an aggressive variant of late-stage metastatic castrate resistant (mCRPC) prostate cancer that commonly arises through neuroendocrine transdifferentiation (NEtD). Treatment options are limited, ineffective, and for most patients, results in death in less than a year. We previously developed a first-in-field patient-derived xenograft (PDX) model of NEtD. Longitudinal deep transcriptome profiling of this model enabled monitoring of dynamic transcriptional changes during NEtD and in the context of androgen deprivation. Long non-coding RNA (lncRNA) are implicated in cancer where they can control gene regulation. Until now the expression of lncRNAs during NEtD and their clinical associations were unexplored.
We implemented a next-generation sequence analysis pipeline that can detect transcripts at low expression levels and built a genome-wide catalogue (n=37,749) oflncRNAs. We applied this pipeline to 927 clinical samples and our high fidelity NEtD model LTL331 and identified 821 lncRNAs in NEPC. Among these are 122 lncRNAs that robustly distinguish NEPC from prostate adenocarcinoma (AD) patient tumours. The highest expressed lncRNAs within this signature are H19, LINC00617, and SSTR5-AS1. Another 742 are associated with the NEtD process and fall into four distinct patterns of expression (NEtD lncRNA Class I, II, III, and IV) in our PDX model and clinical samples. Each class has significant (z-scores>2) and unique enrichment for transcription factor binding site (TFBS) motifs in their sequences. Enriched TFBS include (1) TP53 and BRN1 in Class I, (2) ELF5, SPIC, and HOXD1 in Class II, (3) SPDEF in Class III, (4) HSF1 and FOXA1 in Class IV, and (5) TWIST1 when merging Class III with IV. Common TFBS in all NEtD lncRNA were also identified and include, E2F, REST, PAX5, PAX9, and STAF. Interrogation of the top deregulated candidates (n=100) in radical prostatectomy adenocarcinoma samples with long-term follow-up (median 18 years) revealed significant clinicopathological associations. Specifically, we identified 25 that are associated with rapid metastasis following androgen deprivation therapy (ADT). Two of these lncRNAs (SSTR5-AS1 and LINC00514) stratified patients undergoing ADT based on patient outcome.
To date, a comprehensive characterization of the dynamic landscape of lncRNAs during the NEtD process has not been performed. A temporal analysis of the PDXbased NEtD model has for the first time provided this dynamic landscape. TFBS analysis identified NEPC-related TF motifs present within the NEtD lncRNA sequences, suggesting functional roles for these lncRNAs in NEPC pathogenesis. Furthermore, select NEtD lncRNAs appear to be associated with metastasis and patients receiving ADT. Treatment-related metastasis is a clinical consequence of NEPC tumours. Top candidate lncRNAs FENDRR, H19, LINC00514, LINC00617, and SSTR5-AS1 identified in this study are implicated in the development of NEPC. We present here for the first time a genome-wide catalogue of NEtD lncRNAs that characterize the transdifferentiation process and a robust NEPC lncRNA patient expression signature. To accomplish this, we carried out the largest integrative study that applied a PDX NEtD model to clinical samples. These NEtD and NEPC lncRNAs are strong candidates for clinical biomarkers and therapeutic targets and warrant further investigation.

Contact Submitter

Additional information:

https://github.com/HicServices/RDMP

https://www.dundee.ac.uk/hic/

Accessions (data included in GigaDB):

GEO: GSE62116
BioProject: PRJEB21092
BioProject: PRJEB9660
BioProject: PRJEB19256
GEO: GSE46691

Keywords:

neuroendocrine prostate cancer transdifferentiation small cell carcinoma long non-coding rna 

Transcriptomic, Epigenomic, Genomic, Software

http://gigadb.org/images/data/cropped/100443.png

Funding:

  • Funding body - Terry Fox Foundation
  • Award ID - 201012TFF
  • Comment - Terry Fox Foundation New Frontiers Program on Prostate Cancer
  • Awardee - Colin C Collins
  • Funding body - Mitacs
  • Award ID - IT04310
  • Comment - Mitacs Accelerate PhD Fellowship Program
  • Awardee - Varune R Ramnarine
  • Funding body - Prostate Cancer Team Grant
  • Award ID - T2013-01
  • Comment - Prostate Cancer Canada Team Grant
  • Awardee - Colin C Collins

Samples: Table Settings

Columns:

Common Name
Scienfic Name
Sample Attributes
Taxonomic ID
Genbank Name

Sample IDTaxonomic IDCommon NameGenbank NameScientific NameSample Attributes
V879606HumanhumanHomo sapiens Species Name:Homo sapiens
Description:lncRNA from human adenocarcinoma-neoad...
Reference for biomaterial:DOI:10.1186/s13059-014-0...
...
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V889606HumanhumanHomo sapiens Species Name:Homo sapiens
Description:lncRNA from human adenocarcinoma-neoad...
Reference for biomaterial:DOI:10.1186/s13059-014-0...
...
+
V899606HumanhumanHomo sapiens Species Name:Homo sapiens
Description:lncRNA from human adenocarcinoma (AD-...
Reference for biomaterial:DOI:10.1186/s13059-014-0...
...
+
V99606HumanhumanHomo sapiens Species Name:Homo sapiens
Description:lncRNA from human adenocarcinoma-neoad...
Reference for biomaterial:Current Study
...
+
V909606HumanhumanHomo sapiens Species Name:Homo sapiens
Description:lncRNA from human adenocarcinoma-neoad...
Reference for biomaterial:Current Study
...
+
V919606HumanhumanHomo sapiens Species Name:Homo sapiens
Description:lncRNA from human adenocarcinoma-neoad...
Reference for biomaterial:DOI:10.1002/path.4047
...
+
V929606HumanhumanHomo sapiens :Homo sapiens
Description:lncRNA from human adenocarcinoma-neoad...
Reference for biomaterial:DOI:10.1002/path.4047
...
+
Displaying 121-127 of 127 Sample(s).

Files: (FTP site) Table Settings

Columns:

File Description
Sample ID
Data Type
File Format
Size
Release Date
Download Link
File Attributes

File NameSample IDData TypeFile FormatSizeRelease Date 
mixed archiveTAR251.29 GB2018-05-08
mixed archiveTAR126.76 GB2018-05-08
mixed archiveTAR969.85 MB2018-05-08
mixed archiveTAR380.3 MB2018-05-08
mixed archiveTAR1.14 GB2018-05-08
mixed archiveTAR4.33 GB2018-05-08
annotationFASTA2.93 GB2018-05-08
annotationUNKNOWN808.65 MB2018-05-08
annotationUNKNOWN808.62 MB2018-05-08
annotationUNKNOWN383.29 KB2018-05-08
Displaying 1-10 of 14 File(s).

History:

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